Lee Philip, Gaige Terry, Hung Paul
CellASIC Corporation, Hayward, California, USA.
Methods Cell Biol. 2011;102:77-103. doi: 10.1016/B978-0-12-374912-3.00004-3.
Microfluidic systems provide many advantages for live cell imaging, including improved cell culture micro-environments, control of flows and dynamic exposure profiles, and compatibility with existing high resolution microscopes. Here, we will discuss our approach for design and engineering of microfluidic cell culture environments as well as interfacing with standard laboratory tools and protocols. We focus on an application specific design concept, whereby a shared fabrication process is used to deliver multiple products for different biological applications. As adoption of advanced in vitro models increases, we envision the use of microfluidic cell culture technology to become commonplace.
微流控系统为活细胞成像提供了诸多优势，包括改善细胞培养微环境、控制流体流动和动态暴露模式，以及与现有的高分辨率显微镜兼容。在此，我们将讨论我们设计和构建微流控细胞培养环境的方法，以及与标准实验室工具和方案的对接。我们专注于一种特定应用的设计理念，即使用共同的制造工艺来生产适用于不同生物学应用的多种产品。随着先进体外模型的应用日益增加，我们预计微流控细胞培养技术将变得司空见惯。
