Kim So Rim, Lee Seul Ki, Jang Soo Hwa, Choi Jae-Hong, Lee Byung-Cheon, Hwang In Koo, Lee So Yeong, Ryu Pan Dong
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute fo Veterinary Science, Seoul National University, Korea.
J Acupunct Meridian Stud. 2011 Jun;4(2):102-6. doi: 10.1016/S2005-2901(11)60015-0.
The primo-vascular system is described as the anatomical structure corresponding to acupuncture meridians and has been identified in several tissues in the body, but its detailed anatomy and physiology are not well understood. Recently, the presence of keratin 10 (Krt10) in primo-vascular tissue was reported, but this finding has not yet been confirmed. In this study, we compared Krt10 expression in primo-vascular tissues located on the surface of rat abdominal organs with Krt10 expression on blood and lymphatic vessels. Krt10 protein (approximately 56.5 kDa) was evaluated by western blot analysis and immunohistochemistry. Krt10 (IR) in the primo-node was visualized as patchy spots around each cell or as a follicle-like structure containing a group of cells. Krt10 IR was also identified in vascular and lymphatic tissues, but its distribution was diffuse over the extracellular matrix of the vessels. Thus Krt10 protein was expressed in all three tissues tested, but the expression pattern of Krt10 in primo-vascular tissue differed from those of blood and lymphatic vascular tissues, suggesting that structural and the regulatory roles of Krt10 in primo-vascular system are different from those in blood and lymphatic vessels.
初级血管系统被描述为与针灸经络相对应的解剖结构，已在人体的多种组织中被识别出来，但其详细的解剖学和生理学仍未被充分理解。最近，有报道称初级血管组织中存在角蛋白10（Krt10），但这一发现尚未得到证实。在本研究中，我们比较了大鼠腹部器官表面初级血管组织中Krt10的表达与血管和淋巴管中Krt10的表达。通过蛋白质免疫印迹分析和免疫组织化学评估Krt10蛋白（约56.5 kDa）。初级淋巴集结中的Krt10（免疫反应性）表现为每个细胞周围的斑点状或包含一组细胞的滤泡样结构。在血管和淋巴组织中也鉴定出了Krt10免疫反应性，但其分布在血管的细胞外基质中较为弥散。因此，Krt10蛋白在所有三种测试组织中均有表达，但Krt10在初级血管组织中的表达模式与血管和淋巴管组织不同，这表明Krt10在初级血管系统中的结构和调节作用与在血管和淋巴管中的不同。
