Jia Zhao-Feng, Lee Byung-Cheon, Eom Ki-Hoon, Cha Jin-Myung, Lee Jin-Kyu, Su Zhen-Dong, Yu Wen-Hui, Ryu Pan Dong, Soh Kwang-Sup
Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University, 599 Gwanak, Gwanak-gu, Seoul, Korea.
J Acupunct Meridian Stud. 2010 Sep;3(3):150-5. doi: 10.1016/S2005-2901(10)60030-1.
The primo vascular system was found in the epineurium along the rat sciatic nerve following subcutaneous injection of fluorescent nanoparticles at the Zusanli acupoint (ST-36). Nanoparticles were injected into the primo-vessel near ST-36 and flowed along the sciatic nerve. Fluorescence revealed a structure in the epineurium that was hardly detectable. Images of the isolated sample stained with 4',6-diamidino-2-phenylindole were captured using confocal microscopy. These images showed the distinctive nuclei distribution and multi-lumen structure of primo-vessels that differentiate them from lymphatic vessels, blood capillaries and nerves. This study demonstrates a new use for nanoparticles in fluorescence reflectance imaging techniques during in vivo imaging of primo-vessels.
在足三里穴（ST-36）皮下注射荧光纳米颗粒后，在大鼠坐骨神经的神经外膜中发现了初级血管系统。纳米颗粒被注入靠近ST-36的初级血管，并沿着坐骨神经流动。荧光显示出神经外膜中一个难以检测到的结构。使用共聚焦显微镜拍摄了用4',6-二脒基-2-苯基吲哚染色的分离样本的图像。这些图像显示了初级血管独特的细胞核分布和多腔结构，这使其与淋巴管、毛细血管和神经区分开来。本研究证明了纳米颗粒在初级血管体内成像的荧光反射成像技术中的新用途。
