Lim Chae Jeong, Shen Yiming, Choi Min Cheol, Ryu Pan Dong
Department of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute of Veterinary Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Medical Imaging, College of Veterinary Medicine and Research Institute of Veterinary Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
J Acupunct Meridian Stud. 2020 Aug;13(4):136-145. doi: 10.1016/j.jams.2020.07.002. Epub 2020 Aug 6.
The primo vascular system (PVS) is a novel network composed of primo nodes (PNs) and primo vessels (PVs). Currently, its anatomy is not fully understood.
The aim of this study was to elucidate the three-dimensional PN-PV structure.
Organ-surface PVS tissue was isolated from healthy and anemic rats. The tissues were analyzed by X-ray microcomputed tomography (CT), hematoxylin and eosin staining, and scanning electron microscopy.
From CT images, we identified one or more bundles in a PV. In the PN, the bundles were enlarged and existed in isolation and/or in anastomosis. The transverse CT images revealed four areas of distinct intensities: zero, low, intermediate, and high. The first two were considered to be the sinuses and the subvessels of the PVS and were identified in the hematoxylin and eosin-stained PN sections. The enlargement of the PN from anemic rats was associated with an increase in the intermediate-intensity area. The high-intensity area demarcated the bundle and was overlapped with the mesothelial cells. In scanning electron microscopy, the PV bundles branched out, tapering down to a single bundle at some distance from the PN. Each bundle was composed of several subvessels (∼5 μm). Clustered round microcells (1-25 μm), scattered flat oval cells (∼15 μm), and amorphous extracellular matrix were observed on the surface of the PVS tissue.
The results newly showed that the primo bundle is a structural unit of both PVs and PNs. A bundle was demarcated by high CT intensity and mesothelial cells and consisted of multiple subvessels. The PN bundles contained also sinuses.
原血管系统（PVS）是一个由原节点（PNs）和原血管（PVs）组成的新型网络。目前，其解剖结构尚未完全明确。
本研究旨在阐明三维的PN - PV结构。
从健康和贫血大鼠中分离出器官表面的PVS组织。通过X射线显微计算机断层扫描（CT）、苏木精和伊红染色以及扫描电子显微镜对组织进行分析。
从CT图像中，我们在一个PV中识别出一个或多个束状结构。在PN中，这些束状结构扩大并单独存在和/或相互吻合。横向CT图像显示出四个不同强度的区域：零、低、中、高。前两个区域被认为是PVS的窦和亚血管，并在苏木精和伊红染色的PN切片中得到确认。贫血大鼠的PN扩大与中等强度区域的增加有关。高强度区域划定了束状结构，并与间皮细胞重叠。在扫描电子显微镜下，PV束状结构分支，在距PN一定距离处逐渐变细为单个束状结构。每个束状结构由几个亚血管（约5μm）组成。在PVS组织表面观察到聚集的圆形微细胞（1 - 25μm）、散在的扁平椭圆形细胞（约15μm）和无定形细胞外基质。
结果新显示原束是PVs和PNs的结构单位。一个束状结构由高CT强度和间皮细胞划定，由多个亚血管组成。PN束状结构中也含有窦。
