用免疫荧光抗独特型抗体检测白色念珠菌酵母杀伤毒素细胞壁受体

Detection by immunofluorescent anti-idiotypic antibodies of yeast killer toxin cell wall receptors of Candida albicans.

作者信息

Polonelli L, Fanti F, Conti S, Campani L, Gerloni M, Castagnola M, Morace G, Chezzi C

机构信息

Istituto di Microbiologia, Università degli Studi di Parma, Italy.

出版信息

J Immunol Methods. 1990 Sep 14;132(2):205-9. doi: 10.1016/0022-1759(90)90031-p.

DOI:10.1016/0022-1759(90)90031-p

PMID:2170534

Abstract

Yeast killer toxin cell wall receptors of Candida albicans were observed by indirect immunofluorescence using an affinity purified rabbit anti-idiotypic antiserum. The antiserum had been raised against a monoclonal antibody neutralizing the in vitro activity of a killer toxin produced by a selected strain of Hansenula anomala UCSC 25F. This simple procedure permitted the location of killer toxin cell wall receptors in various morphological phases of the yeast cells. The use of the indirect immunofluorescence technique with anti-idiotypic antibodies may have potential value in determining the occurrence of killer toxin receptors in other microbial systems.

摘要

利用亲和纯化的兔抗独特型抗血清，通过间接免疫荧光观察白色念珠菌的酵母杀伤毒素细胞壁受体。该抗血清是针对一种单克隆抗体产生的，该单克隆抗体可中和由异常汉逊酵母UCSC 25F选定菌株产生的杀伤毒素的体外活性。这个简单的程序可以确定酵母细胞不同形态阶段中杀伤毒素细胞壁受体的位置。使用抗独特型抗体的间接免疫荧光技术在确定其他微生物系统中杀伤毒素受体的存在方面可能具有潜在价值。