利用单克隆抗体对酵母杀伤毒素进行血清学研究。

Serological study of yeast killer toxins by monoclonal antibodies.

作者信息

Polonelli L, Manzara S, Conti S, Dettori G, Morace G, Chezzi C

机构信息

Istituto di Microbiologia, Universitá di Parma, Italy.

出版信息

Mycopathologia. 1989 Dec;108(3):211-5. doi: 10.1007/BF00436228.

DOI:10.1007/BF00436228

PMID:2515441

Abstract

Yeast killer toxins coded by determined and undetermined killer plasmids or presumptive nuclear gene(s) in various genera (Saccharomyces, Kluyveromyces, Pichia and Candida) have been serologically investigated by a monoclonal antibody (KT4), produced against the yeast killer toxin of Pichia (Hansenula) anomala UCSC 25F. Double immunodiffusion with the killer toxins as antigens and indirect immunofluorescence on whole cells of the corresponding killer yeast have been used. In both the serological procedures, monoclonal antibody KT4 proved to be reacting only with the killer toxins and the whole cells of yeasts belonging to the genus Pichia.

摘要

已通过针对异常毕赤酵母（汉逊酵母）UCSC 25F酵母杀伤毒素产生的单克隆抗体（KT4），对各种属（酿酒酵母属、克鲁维酵母属、毕赤酵母属和念珠菌属）中由确定和不确定的杀伤质粒或假定核基因编码的酵母杀伤毒素进行了血清学研究。使用了以杀伤毒素为抗原的双向免疫扩散以及对相应杀伤酵母全细胞的间接免疫荧光法。在这两种血清学方法中，单克隆抗体KT4均被证明仅与毕赤酵母属酵母的杀伤毒素和全细胞发生反应。