Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
Plant Cell. 2011 Jun;23(6):2143-54. doi: 10.1105/tpc.111.083436. Epub 2011 Jun 24.
Because plant reproductive development occurs only in adult plants, the juvenile-to-adult phase change is an indispensable part of the plant life cycle. We identified two allelic mutants, peter pan syndrome-1 (pps-1) and pps-2, that prolong the juvenile phase in rice (Oryza sativa) and showed that rice PPS is an ortholog of Arabidopsis thaliana CONSTITUTIVE PHOTOMORPHOGENIC1. The pps-1 mutant exhibits delayed expression of miR156 and miR172 and the suppression of GA biosynthetic genes, reducing the GA(3) content in this mutant. In spite of its prolonged juvenile phase, the pps-1 mutant flowers early, and this is associated with derepression of RAP1B expression in pps-1 plants independently of the Hd1-Hd3a/RFT1 photoperiodic pathway. PPS is strongly expressed in the fourth and fifth leaves, suggesting that it regulates the onset of the adult phase downstream of MORI1 and upstream of miR156 and miR172. Its ability to regulate the vegetative phase change and the time of flowering suggests that rice PPS acquired novel functions during the evolution of rice/monocots.
由于植物的生殖发育仅发生在成年植物中，因此幼年期到成年期的转变是植物生命周期中不可缺少的一部分。我们鉴定了两个等位突变体，彼得潘综合征-1（pps-1）和 pps-2，它们延长了水稻（Oryza sativa）的幼年期，并表明水稻 PPS 是拟南芥 CONSTITUTIVE PHOTOMORPHOGENIC1 的同源物。pps-1 突变体表现出 miR156 和 miR172 的表达延迟以及 GA 生物合成基因的抑制，从而降低了该突变体中的 GA(3)含量。尽管其幼年期延长，但 pps-1 突变体仍早期开花，这与 pps-1 植物中 RAP1B 表达的去阻遏有关，而与 Hd1-Hd3a/RFT1 光周期途径无关。PPS 在第四和第五叶片中强烈表达，表明它在 MORI1 下游和 miR156 和 miR172 上游调节成年期的开始。它调节营养生长阶段变化和开花时间的能力表明，在水稻/单子叶植物的进化过程中，水稻 PPS 获得了新的功能。
