干扰素-γ释放试验（QuantiFERON-TB Gold® In Tube）在儿童结核病中的诊断价值。
Diagnostic value of interferon- gamma release assays (QuantiFERON-TB Gold® In Tube) in childhood tuberculosis.
机构信息
Department of Pediatrics, S.N. Medical College, Agra, India.
出版信息
Indian J Pediatr. 2012 Feb;79(2):183-7. doi: 10.1007/s12098-011-0469-y. Epub 2011 Jun 25.
OBJECTIVE
To evaluate the efficacy and diagnostic potential of interferon- gamma release assays QuantiFERON-TB Gold® In Tube (QFT-TB-IT) for the detection of gamma interferon against the antigen ESAT-6, CFP-10, TB 7.7(p4) of Mycobacterium tuberculosis and compare the reactivity with other methods (smear, culture and IS6110 targeting PCR).
METHODS
The study was carried out on children with pulmonary and extra pulmonary tuberculosis along with matching and PPD+ controls (82 cases, 48 matching controls and 20 PPD+ controls).
RESULTS
QFT-TB-IT showed a significantly higher sensitivity (51.2%) as compared with LJ medium culture method (11.0%; p < 0.001), BacT/Alert 3D system (12.1%; p < 0.001), ZN staining (19.5%; p < 0.001) and PCR (45.1%; p < 0.05). Specificity of QFT-TB-IT was 48.0%.
CONCLUSIONS
Less specificity of this assay may be due to high endemicity of tuberculosis. However, further studies are recommended.
目的
评估干扰素-γ释放试验 QuantiFERON-TB Gold® In Tube（QFT-TB-IT）针对结核分枝杆菌抗原 ESAT-6、CFP-10、TB 7.7(p4) 检测γ干扰素的功效和诊断潜力，并与其他方法（涂片、培养和针对 IS6110 的 PCR）的反应性进行比较。
方法
该研究在患有肺内和肺外结核的儿童以及匹配和 PPD+对照者（82 例，48 例匹配对照者和 20 例 PPD+对照者）中进行。
结果
QFT-TB-IT 的敏感性（51.2%）显著高于 LJ 培养基培养法（11.0%；p<0.001）、BacT/Alert 3D 系统（12.1%；p<0.001）、ZN 染色（19.5%；p<0.001）和 PCR（45.1%；p<0.05）。QFT-TB-IT 的特异性为 48.0%。
结论
该检测方法的特异性较低可能是由于结核病的高地方性。然而，建议进行进一步的研究。
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