Tissue Engineering Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology, Amagasaki site, Amagasaki, Hyogo, Japan.
J Tissue Eng Regen Med. 2012 Apr;6(4):261-71. doi: 10.1002/term.428. Epub 2011 Jun 27.
Bone marrow-derived mesenchymal stem cells (BMSCs) and adipose tissue-derived mesenchymal stem cells (AMSCs) have been used clinically for tissue regeneration; however, their proliferation/differentiation potentials are limited. Recently, induced pluripotent stem cells (iPSCs), known to have nearly unlimited potential to proliferate and differentiate into cells of all three germ layers, have gained wide interest in regenerative medicine. Here, we generated iPSCs from frozen-stocked AMSCs and BMSCs and examined their biological characteristics by comparative analyses. Although the iPSCs were more challenging to generate from the BMSCs than the AMSCs, both iPSC populations expressed pluripotent markers, such as stage-specific embryonic antigen (SSEA)-3, SSEA-4, tumour-related antigens (TRAs) TRA-1-60 and TRA-1-81, OCT3/4 and NANOG. Furthermore, both cell populations differentiated well into three germ layer-derived cells, both in vitro and in vivo. These results indicate that iPSCs derived from frozen AMSCs/BMSCs exhibit equally acceptable iPSC characteristics and have potential in clinical applications as an alternative source of autogenous stem cells.
骨髓间充质干细胞（BMSCs）和脂肪组织来源的间充质干细胞（AMSCs）已在临床上用于组织再生；然而，它们的增殖/分化潜力有限。最近，诱导多能干细胞（iPSCs），已知具有几乎无限的增殖和分化为三个胚层细胞的潜力，在再生医学中引起了广泛的关注。在这里，我们从冷冻保存的 AMSCs 和 BMSCs 中生成 iPSCs，并通过比较分析来研究它们的生物学特性。尽管从 BMSCs 中生成 iPSCs 比从 AMSCs 中更具挑战性，但两种 iPSC 群体都表达了多能标记物，如阶段特异性胚胎抗原（SSEA）-3、SSEA-4、肿瘤相关抗原（TRA）TRA-1-60 和 TRA-1-81、OCT3/4 和 NANOG。此外，两种细胞群体都能很好地在体外和体内分化为三个胚层来源的细胞。这些结果表明，源自冷冻 AMSCs/BMSCs 的 iPSCs 表现出同样可接受的 iPSC 特征，并具有作为自体干细胞替代来源在临床应用中的潜力。
