Takagi Hirotaka, Sugiyama Kazuyoshi
Division of Biological Safety Control and Research, National Institute of Infectious Diseases.
Kansenshogaku Zasshi. 2011 May;85(3):244-9. doi: 10.11150/kansenshogakuzasshi.85.244.
Titanium dioxide (TiO2) photocatalysis causes oxidative destruction dependent on electrons excited by < or = 400 nm ultraviolet (UV) rays. Many studies have covered the destruction of organics and bacteria and bacteriophage inactivation by photocatalysis. We studied the inactivation by new nonwoven siliconized titanium dioxide fabric of the feline calicivirus F9 (FCV-F9), human adenovirus GB (HAdv3-GB), and influenza A and B virus (A/New Caledonia, B/Shandong, and 5 clinical strains). We spotted 10 microL of viral suspensions containing infectious 5 log10 50% tissue culture doses (TCID50) onto 1 cm2 pieces of TiO2-coated nonwoven control fabric treated or not treated with UV light (lambda(max), 365 nm, 1,100-1,300 microW/cm2). We then measured the virus titers of 50 microL of viral suspension recovered from these fabrics. FCV-F9 and HAdv3-GB infectivity titers were reduced by over 3.5 log10 TCID50 after 30 min of irradiation, but influenza viral titer was reduced to where it was undetectable even without UV irradiation. Comparing individual viral titer reduction due to nonwoven fabric contact without UV irradiation exposure, showed that FCV-F9 and HAdv3-GB titer infectivity was not reduced. In contrast, influenza A and B titer infectivity was reduced to 2 log10 TCID50 after 5 min of contact with the nonwoven fabric and to 3 log10 TCID50 after 30 min of contact. Titers of 6 of 7 influenza A and B strains were reduced by over 4 log10 TCID50 within 30 min. Siliconized TiO2-coated nonwoven fabric thus efficiently inactivated FCV-F9 and HAdV-GB and absorbed influenza viruses.
二氧化钛（TiO₂）光催化作用会引发依赖于波长小于或等于400纳米紫外线（UV）激发电子的氧化破坏反应。许多研究都涉及了光催化对有机物的破坏以及对细菌和噬菌体的灭活作用。我们研究了新型非织造硅化二氧化钛织物对猫杯状病毒F9（FCV-F9）、人腺病毒GB（HAdv3-GB）以及甲型和乙型流感病毒（A/新喀里多尼亚、B/山东以及5株临床分离株）的灭活效果。我们将含有5 log₁₀ 50%组织培养感染剂量（TCID₅₀）的10微升病毒悬液点涂在1平方厘米经或未经波长为365纳米、光强为1100 - 1300微瓦/平方厘米紫外线照射处理的TiO₂涂层非织造对照织物上。然后我们测量了从这些织物上回收的50微升病毒悬液的病毒滴度。照射30分钟后，FCV-F9和HAdv3-GB的感染性滴度降低了超过3.5 log₁₀ TCID₅₀，但即使没有紫外线照射，流感病毒滴度也降低到无法检测的水平。比较未经紫外线照射仅因非织造织物接触导致的单个病毒滴度降低情况，发现FCV-F9和HAdv3-GB的滴度感染性并未降低。相比之下，甲型和乙型流感病毒与非织造织物接触5分钟后，滴度感染性降低到2 log₁₀ TCID₅₀，接触30分钟后降低到3 log₁₀ TCID₅₀。7株甲型和乙型流感病毒株中的6株在30分钟内滴度降低超过4 log₁₀ TCID₅₀。因此硅化TiO₂涂层非织造织物能有效灭活FCV-F9和HAdV-GB，并吸附流感病毒。
