University Lille 2, Faculty of Medicine, CHRU Lille Laboratory of Virology EA3610, Lille, France.
Intervirology. 2013;56(4):224-30. doi: 10.1159/000350556. Epub 2013 Jun 13.
To evaluate the effect of a disinfectant onto viruses in suspension on the one hand and applied onto a surface on the other.
A system combining flocked swabs to recover viruses dried onto stainless steel carriers and gel filtration to eliminate cytotoxic products has been developed to study the virucidal effect of a quaternary ammonium-based disinfectant towards herpes simplex virus type 1 (HSV-1), coxsackievirus B4 (CVB4) and feline calicivirus F9 (FCV). The recovery of FCV has been estimated by RT real-time PCR.
HSV-1, CVB4 and FCV had a titer over 10(4) TCID50 · ml(-1) after 2 h drying and were recovered from the carriers using flocked swabs. HSV-1 was inactivated in suspension and on stainless steel carriers by the disinfectant (a reduction factor of 4 and 2.83 log, respectively) whereas CVB4 was resistant. The reduction of infectious titer was moderate, 1.5 log in 30 min, when FCV was in suspension, whereas it was up to 4 log in 10 min when the virus was dried on a carrier. Dried FCV was efficiently recovered from carriers as demonstrated by RT real-time PCR.
A non-enveloped virus, FCV, applied on a surface, but not in suspension, was inactivated by a quaternary ammonium-based disinfectant. The resistance of viruses applied onto a surface to the effect of disinfectants should be investigated further.
一方面评估消毒剂对悬浮病毒的效果，另一方面评估消毒剂对表面病毒的效果。
为了研究一种季铵盐消毒剂对单纯疱疹病毒 1 型（HSV-1）、柯萨奇病毒 B4（CVB4）和猫杯状病毒 F9（FCV）的病毒杀灭效果，开发了一种组合使用植绒拭子回收干燥在不锈钢载体上的病毒和凝胶过滤以消除细胞毒性产物的系统。通过 RT 实时 PCR 来估计 FCV 的回收率。
在干燥 2 小时后，HSV-1、CVB4 和 FCV 的滴度超过 10(4)TCID50·ml(-1)，并可使用植绒拭子从载体上回收。HSV-1 在悬浮液中和不锈钢载体上均被消毒剂灭活（分别减少 4 和 2.83 个对数），而 CVB4 具有抗性。当 FCV 悬浮时，其传染性滴度在 30 分钟内降低 1.5 个对数，而当病毒干燥在载体上时，其降低高达 4 个对数。如 RT 实时 PCR 所示，干燥的 FCV 可从载体上有效回收。
非包膜病毒 FCV 应用于表面（而非悬浮液中）时，会被季铵盐消毒剂灭活。应进一步研究应用于表面的病毒对消毒剂效果的抗性。
