Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, Japan.
Laboratory of Biometabolic Chemistry, School of Health Sciences, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan.
Int J Nanomedicine. 2020 Feb 28;15:1387-1395. doi: 10.2147/IJN.S229880. eCollection 2020.
A previous study demonstrated the virucidal effect of an electrically charged disinfectant (CAC-717), which contains meso-structure nanoparticles, on enveloped viruses (influenza viruses). However, the effect of CAC-717 on other microorganisms and the mechanisms by which CAC-717 inactivates the microorganisms remain unclear. In this study, CAC-717 was further evaluated in terms of its biocidal and virucidal activity as well as its effect on bacterial and viral nucleic acids.
The inactivation effects of CAC-717 against various microorganisms [non-enveloped virus, feline calicivirus (FCV); bacteria, and ] were investigated by comparing the viral titer of the medium tissue culture infectious dose (TCID) and the value (estimated treatment time required to reduce the number of microorganisms by 90%). Furthermore, the effects of CAC-717 on viral and bacterial genomic RNA/DNA were examined using a polymerase chain reaction (PCR).
Treatment of an equal volume of CAC-717 with cell lysate infected with a non-enveloped virus, feline calicivirus (FCV), reduced the TCID. Viral titer dropped below the detection limit after 2 min of treatment. The value of FCV was 0.256 min (average of multiple endpoint values) and endpoint value was 0.341 min. The value for and was 0.290 min and 0.080 min (average of multiple endpoint values), respectively and the endpoint value was 0.545 min and 0.054 min, respectively. In addition, PCR showed the inhibition of nucleic acid amplification of the RNA and DNA genome of FCV and bacteria, respectively.
Our findings suggest that CAC-717 inactivates viruses and bacteria by modifying the viral and bacterial nucleic acids.
先前的一项研究表明，一种带电荷的消毒剂（CAC-717）具有杀病毒作用，其中包含介孔结构纳米颗粒，可杀灭包膜病毒（流感病毒）。然而，CAC-717 对其他微生物的作用以及 CAC-717 使微生物失活的机制尚不清楚。在这项研究中，进一步评估了 CAC-717 的杀菌和杀病毒活性以及对细菌和病毒核酸的影响。
通过比较介质组织培养感染剂量（TCID）和 值（估计减少微生物数量所需的处理时间减少 90%），研究了 CAC-717 对各种微生物（非包膜病毒、猫杯状病毒（FCV）；细菌）的灭活作用。此外，使用聚合酶链反应（PCR）检查 CAC-717 对病毒和细菌基因组 RNA/DNA 的影响。
用细胞裂解液处理与非包膜病毒（猫杯状病毒（FCV））感染的等体积 CAC-717，降低了 TCID。处理 2 分钟后，病毒滴度降至检测限以下。FCV 的 值为 0.256 分钟（多个终点 值的平均值），终点 值为 0.341 分钟。 和 的 值分别为 0.290 分钟和 0.080 分钟（多个终点 值的平均值），终点 值分别为 0.545 分钟和 0.054 分钟。此外，PCR 显示分别抑制了 FCV 和细菌的 RNA 和 DNA 基因组的核酸扩增。
我们的研究结果表明，CAC-717 通过修饰病毒和细菌的核酸来灭活病毒和细菌。
