Department of Pharmaceutical Engineering, Tianjin University of Commerce, Tianjin, China.
Nat Prod Res. 2012;26(5):479-83. doi: 10.1080/14786419.2010.516433. Epub 2011 Jun 27.
In this study, the composition of fatty oil from Semen Ziziphi Spinosae and its cardiotonic activity on the heart isolated from a toad were studied. Oil-in-water (O/W) emulsions of fatty oil were prepared by the perfusion method. The fatty oil had a positive inotropic effect on isolated rat hearts at a concentration between 5 × 10(-3) and 2 × 10(-2) mL/10 mL, and the effect was in positive correlation with the concentration of calcium ions. In addition, this effect was inhibited by 2 mg/mL nifedipine, suggesting that the cardiotonic mechanism could be responsible for accelerating the inflow of calcium ions. Gas chromatography-mass spectrometry analysis showed that the main constituents of the fatty oil were 9-octadecenoic acid (43.32%), 9,12-octadecadienoic acid (42.57%), hexadecanoic acid (4.76%), 9-eicosenoic acid (2.95%), stearic acid (2.41%) and arachidic acid (0.81%). This preliminary study revealed that the fatty oil of Semen Ziziphi Spinosae exhibited remarkable cardiotonic activity in the tested models, and it is necessary to further reveal the effective substances of the fatty oil.
本研究以酸枣仁脂肪油为研究对象，探讨其组成成分及其对蟾蜍离体心脏的强心作用。采用灌注法制备脂肪油的油包水乳剂。脂肪油在 5×10(-3)至 2×10(-2)mL/10mL 浓度范围内对离体大鼠心脏具有正性变力作用，且作用与钙离子浓度呈正相关。此外，该作用被 2mg/mL 硝苯地平抑制，提示其强心机制可能与加速钙离子内流有关。气相色谱-质谱联用分析表明，脂肪油的主要成分为 9-十八碳烯酸（43.32%）、9,12-十八碳二烯酸（42.57%）、十六烷酸（4.76%）、9-二十碳烯酸（2.95%）、硬脂酸（2.41%）和花生酸（0.81%）。本初步研究表明，酸枣仁脂肪油在受试模型中表现出显著的强心作用，有必要进一步揭示其有效物质。
