National University of Singapore, 117576 Singapore.
Nanomedicine (Lond). 2011 Aug;6(6):961-74. doi: 10.2217/nnm.11.26. Epub 2011 Jun 27.
Using scaffolds with autologous stem cells is a golden strategy for the treatment of bone defects. In this strategy, human mesenchymal stem cells (hMSCs) have often been isolated and expanded in vitro on a plastic surface to obtain a sufficient cell number before seeding on a suitable scaffold.
MATERIALS & METHODS: Investigating the influence of serial passages (from passage two to passage eight) on the abilities of proliferation and osteogenic differentiation of hMSCs on 24-well tissue culture polystyrene plates and poly L-lactic acid electrospun nanofibrous scaffolds was performed to determine how prolonged culture affected these cellular abilities and how the nanofibrous scaffolds supported the osteogenic differentiation potential of hMSCs.
RESULTS & CONCLUSION: Serial passage caused adverse changes in hMSCs characteristics, which were indicated by the decline in both proliferation and osteogenic differentiation abilities. Interestingly, the poly L-lactic acid nanofibrous scaffolds showed a significant support in recovering the osteogenic abilities of hMSCs, which had been severely affected by prolonged culture.
使用带有自体干细胞的支架是治疗骨缺损的黄金策略。在这种策略中，通常将人骨髓间充质干细胞（hMSCs）在体外分离和扩增在塑料表面上，以在合适的支架上接种之前获得足够的细胞数量。
在 24 孔组织培养聚苯乙烯板和聚 L-乳酸电纺纳米纤维支架上研究了传代（从第二代到第八代）对 hMSCs 的增殖和成骨分化能力的影响，以确定延长培养如何影响这些细胞能力，以及纳米纤维支架如何支持 hMSCs 的成骨分化潜能。
传代导致 hMSCs 特性发生不良变化，这表现为增殖和成骨分化能力下降。有趣的是，聚 L-乳酸纳米纤维支架在恢复 hMSCs 的成骨能力方面表现出显著的支持作用，而延长培养严重影响了 hMSCs 的成骨能力。
