Howard H L, McLaughlin-Taylor E, Hill R L
Animal Resources Center, Beckman Research Institute, City of Hope, Duarte, CA.
Lab Anim Sci. 1990 Sep;40(5):510-4.
The purpose of this study was to determine the effects that specific euthanasia methods have on mitogen induced lymphocyte proliferation (LP) and the induction of alloantigen specific cytolytic T-lymphocytes (CTL). Mice were euthanatized by cervical dislocation (CD), or anesthesia with methoxyflurane or pentobarbital followed by CD (M-CD or P-CD respectively), CO2 overexposure (CO2-OD) or halothane overexposure (H-OD). Mitogenic lymphoproliferation was increased in cells derived from mice euthanatized by M-CD and P-CD. In contrast, the cytolytic profile of CTL derived from mice euthanatized by P-CD, CO2-OD and H-OD was decreased. The results of this study show that euthanasia techniques involving the use of methoxyflurane, pentobarbital, CO2 and halothane affect in vitro lymphoproliferation and CTL function. We conclude that the method of euthanasia influences certain immunologic parameters and selection of a particular technique should be given careful consideration.
本研究的目的是确定特定安乐死方法对丝裂原诱导的淋巴细胞增殖（LP）和同种异体抗原特异性细胞毒性T淋巴细胞（CTL）诱导的影响。通过颈椎脱臼（CD）、用甲氧氟烷或戊巴比妥麻醉后再进行颈椎脱臼（分别为M-CD或P-CD）、过度暴露于CO2（CO2-OD）或过度暴露于氟烷（H-OD）对小鼠实施安乐死。M-CD和P-CD安乐死的小鼠来源的细胞中促有丝分裂淋巴细胞增殖增加。相比之下，P-CD、CO2-OD和H-OD安乐死的小鼠来源的CTL的细胞溶解特性降低。本研究结果表明，涉及使用甲氧氟烷、戊巴比妥、CO2和氟烷的安乐死技术会影响体外淋巴细胞增殖和CTL功能。我们得出结论，安乐死方法会影响某些免疫参数，应仔细考虑选择特定技术。
