Pecaut M J, Smith A L, Jones T A, Gridley D S
Department of Radiation Medicine, Loma Linda University, CA 92354, USA.
Comp Med. 2000 Dec;50(6):595-602.
The major goal was to determine whether variations in the method of CO2 euthanasia would induce significant immunologic differences.
Young adult C57BL/6 mice (n = 40) were euthanized, using four regimens: 70% CO2/30% O2; 70% CO2/30% O2-->100% CO2; 100% CO2-naïve chamber; and 100% CO2 pre-charged chamber. Time to recumbency and euthanasia and body, liver, lung, spleen, and thymus masses were determined. Blood and spleen were further evaluated for leukocyte, lymphocyte, and thrombocyte counts, erythrocyte characteristics, distribution of lymphocyte subpopulations, spontaneous and mitogen-induced blastogenesis, complement activity, and cytokine production.
Time to euthanasia was five- to eightfold longer in mice exposed to 70% CO2/30% O2 than that for any other group. There were slight increases in mean erythrocyte volume (MCV) and mean erythrocyte hemoglobin (MCH) for all groups, compared with those for the 100% CO2 pre-charged group. Circulating cytotoxic T (CD8+) lymphocyte percentages and numbers, and spontaneous blastogenesis of leukocytes in blood and spleen, also were affected by euthanasia method.
The method of CO2 euthanasia can result in significant differences in immunologic/hematologic variables. Thus, consistency in euthanasia procedures may be important in accurate interpretation of research data.
主要目标是确定二氧化碳安乐死方法的差异是否会引发显著的免疫学差异。
使用四种方案对成年C57BL/6小鼠（n = 40）实施安乐死：70%二氧化碳/30%氧气；70%二氧化碳/30%氧气→100%二氧化碳；100%二氧化碳空箱；以及100%二氧化碳预充箱。测定小鼠至侧卧及安乐死的时间以及体重、肝脏、肺、脾脏和胸腺的重量。对血液和脾脏进一步评估白细胞、淋巴细胞和血小板计数、红细胞特征、淋巴细胞亚群分布、自发和丝裂原诱导的母细胞化、补体活性及细胞因子产生情况。
暴露于70%二氧化碳/30%氧气环境下的小鼠至安乐死的时间比其他任何组延长了五至八倍。与100%二氧化碳预充组相比，所有组的平均红细胞体积（MCV）和平均红细胞血红蛋白含量（MCH）均略有增加。安乐死方法还影响循环细胞毒性T（CD8 +）淋巴细胞的百分比和数量，以及血液和脾脏中白细胞的自发母细胞化。
二氧化碳安乐死方法可导致免疫/血液学变量出现显著差异。因此，安乐死程序的一致性对于准确解读研究数据可能很重要。
