Laboratory of Environmental Microbiology, Institute of Microbiology of the ASCR, Praha, Czech Republic.
FEMS Microbiol Ecol. 2011 Oct;78(1):59-69. doi: 10.1111/j.1574-6941.2011.01164.x. Epub 2011 Jul 21.
Changes in the abundance of bacteria and fungi and in the composition of bacterial communities during primary succession were investigated in a brown coal mine deposit area near Sokolov, the Czech Republic, using phospholipid fatty acids analysis, microarray and 16S rRNA gene sequencing. The study considered a chronosequence of sites undergoing spontaneous succession: 6-, 12-, 21- and 45-year-old and a 21-year-old site revegetated with Alnus glutinosa. During succession, organic carbon and the total nitrogen content increased while the pH and the C/N ratio decreased. Microbial biomass and bacterial diversity increased until 21 years and decreased later; bacteria dominated over fungi in the initial and late phases of succession. Bacterial community composition of the 6-year-old site with no vegetation cover largely differed from the older sites, especially by a higher content of Gammaproteobacteria, Cyanobacteria and some Alphaproteobacteria. Bacteria belonging to the genera Acidithiobacillus, Thiobacillus and related taxa, the CO(2) and N(2) fixers, dominated the community at this site. In the later phases, bacterial community development seemed to reflect more the changes in soil nutrient content and pH than vegetation with a decrease of Actinobacteria and an increase of Acidobacteria. The site revegetated with A. glutinosa resembled the 45-year-old primary succession site and exhibited an even lower pH and C/N ratio, indicating that recultivation is able to accelerate soil development.
采用磷脂脂肪酸分析、微阵列和 16S rRNA 基因测序的方法，研究了捷克索科洛夫（Sokolov）附近褐煤矿区原生演替过程中细菌和真菌丰度及细菌群落组成的变化。该研究考虑了一个处于自然演替过程中的时间序列：6 年、12 年、21 年和 45 年的演替点，以及一个用欧洲桤木（Alnus glutinosa）重新造林的 21 年演替点。在演替过程中，有机碳和总氮含量增加，而 pH 值和 C/N 比值降低。微生物生物量和细菌多样性在 21 年内增加，随后减少；在演替的初始和后期阶段，细菌数量超过真菌。无植被覆盖的 6 年演替点的细菌群落组成与较老的演替点有很大不同，特别是具有较高含量的γ变形菌纲（Gammaproteobacteria）、蓝细菌（Cyanobacteria）和一些α变形菌纲（Alphaproteobacteria）。属于嗜酸硫杆菌属（Acidithiobacillus）、硫杆菌属（Thiobacillus）和相关类群、CO2 和 N2 固定菌的细菌在该群落中占主导地位。在后期阶段，细菌群落的发展似乎更多地反映了土壤养分含量和 pH 值的变化，而不是植被的变化，放线菌（Actinobacteria）减少，酸杆菌（Acidobacteria）增加。用欧洲桤木重新造林的演替点与 45 年的原生演替点相似，表现出更低的 pH 值和 C/N 比值，表明重新造林能够加速土壤的发育。
