Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya, Japan.
FEBS Lett. 2011 Jul 21;585(14):2367-71. doi: 10.1016/j.febslet.2011.06.009. Epub 2011 Jun 23.
We identified two DYW subclass pentatricopeptide repeat (PPR) proteins, PpPPR_78 and PpPPR_79, as RNA editing factors in the moss Physcomitrella patens. Disruption of each gene by homologous recombination revealed that PpPPR_78 was involved in RNA editing at the rps14 (rps14-C137) and cox1 (cox1-C755) sites and PpPPR_79 at the nad5-1 (nad5-C598) site in the mitochondrial transcripts. RNA editing defects did not affect transcript patterns of the target genes. Thus, DYW subclass PPR proteins seem to be site-specific trans-acting factors for RNA editing.
我们鉴定出两个 DYW 亚类五肽重复（PPR）蛋白，PpPPR_78 和 PpPPR_79，作为苔藓植物Physcomitrella patens 中的 RNA 编辑因子。通过同源重组破坏每个基因，揭示 PpPPR_78 参与 rps14（rps14-C137）和 cox1（cox1-C755）位点以及 PpPPR_79 在线粒体转录物中的 nad5-1（nad5-C598）位点的 RNA 编辑。RNA 编辑缺陷不影响靶基因的转录模式。因此，DYW 亚类 PPR 蛋白似乎是 RNA 编辑的特异性反式作用因子。
