Am J Bot. 1997 Mar;84(3):336.
Delta 9-tetrahydrocannabinol (THC) localization in glandular trichomes and bracteal tissues of Cannabis, prepared by high pressure cryofixation-cryosubstitution, was examined with a monoclonal antibody-colloidal gold probe by electron microscopy (EM). The antibody detected THC in the outer wall of disc cells during the presecretory cavity phase of gland development. Upon formation of the secretory cavity, the immunolabel detected THC in the disc cell wall facing the cavity as well as the subcuticular wall and cuticle throughout development of the secretory cavity. THC was detected in the fibrillar matrix associated with the disc cell and with this matrix in the secretory cavity. The antibody identified THC on the surface of secretory vesicles, but not in the secretory vesicles. Gold label also was localized in the anticlinal walls between adjacent disc cells and in the wall of dermal and mesophyll cells of the bract. Grains were absent or detected only occasionally in the cytoplasm of disc or other cells of the bract. No THC was detected in controls. These results indicate THC to be a natural product secreted particularly from disc cells and accumulated in the cell wall, the fibrillar matrix and surface feature of vesicles in the secretory cavity, the subcuticular wall, and the cuticle of glandular trichomes. THC, among other chemicals, accumulated in the cuticle may serve as a plant recognition signal to other organisms in the environment.
采用高压冷冻-冷冻置换法制备的大麻腺毛和苞片中的 δ9-四氢大麻酚（THC）定位，通过电子显微镜（EM）用单克隆抗体-胶体金探针进行了检查。在腺毛发育的前分泌腔阶段，该抗体在盘状细胞的外壁中检测到 THC。当分泌腔形成时，免疫标记物在面向腔的盘状细胞壁以及在分泌腔发育过程中的皮下壁和角质层中检测到 THC。在与盘状细胞相关的纤维状基质中和在分泌腔中检测到 THC。该抗体鉴定出在分泌小泡表面上的 THC，但不在分泌小泡内。金标记还定位于相邻盘状细胞之间的垂周壁以及苞片中的表皮和叶肉细胞的壁中。在盘状或其他苞片中的细胞质中，颗粒缺失或仅偶尔检测到。在对照中未检测到 THC。这些结果表明 THC 是一种天然产物，特别是从盘状细胞分泌出来的，并积累在细胞壁、纤维状基质和分泌腔中囊泡的表面特征、皮下壁和腺毛的角质层中。与其他化学物质一起积累在角质层中的 THC 可能作为植物识别信号传递给环境中的其他生物体。
