Kim E S, Mahlberg P G
Department of Biology, Konkuk University, Seoul, Korea.
Mol Cells. 1997 Jun 30;7(3):352-9.
Plastids in lipophilic glandular trichomes of chemically fixed (CF) and high pressure cryofixed-cryosubstituted (HPC-CS) bracteal tissues of Cannabis were examined by transmission electron microscopy. In CF preparations, plastids in disc cells prior to secretory cavity formation possessed several lobed and dilated thylakoid-like features. In glands with secretory cavities, thylakoid-like features aggregated to form reticulate bodies that distended regions of the elongated plastids. Electron-gray inclusions evident on the plastid surface appeared continuous with the reticulate body. Inclusions of similar electron density also appeared in the cell cytoplasm, along the plasma membrane, between the plasma membrane and cell wall facing the cavity, and in the secretory cavity in both CF and HPC-CS preparations. The bilayer structure of membranes of the plastid envelope was evident in HPC-CS but not in CF preparations. In HPC-CS preparations, secretions were evident on the plastid surface and were continuous with those in the plastid through pores in the envelope. This study supports an interpretation that these specialized plastids, lipoplasts, synthesize secretions that are transported through the plasma membrane and cell wall to subsequently accumulate in the secretory cavity.
通过透射电子显微镜检查了化学固定（CF）和高压冷冻固定-冷冻替代（HPC-CS）的大麻苞片组织中亲脂性腺毛中的质体。在CF制剂中，分泌腔形成之前盘状细胞中的质体具有几个叶状和扩张的类囊体样特征。在具有分泌腔的腺体中，类囊体样特征聚集形成网状体，使伸长的质体区域膨胀。质体表面明显的电子灰色内含物似乎与网状体相连。在CF和HPC-CS制剂中，类似电子密度的内含物也出现在细胞质中、沿着质膜、在面向腔的质膜和细胞壁之间以及分泌腔中。质体包膜膜的双层结构在HPC-CS中明显，但在CF制剂中不明显。在HPC-CS制剂中，质体表面有明显的分泌物，这些分泌物通过包膜中的孔与质体中的分泌物相连。这项研究支持这样一种解释，即这些特殊的质体，即脂质体，合成的分泌物通过质膜和细胞壁运输，随后在分泌腔中积累。
