Department of Zoology, Oregon State University, Corvallis, Oregon 97731.
Integr Comp Biol. 2002 Aug;42(4):815-24. doi: 10.1093/icb/42.4.815.
An important step in connecting the organismal response to thermal stress to patterns of community structure is determining at what scale discernable levels of variation are manifested. The temperature signal to which organisms may potentially respond varies at many spatial scales including microhabitat, tidal height, site and latitude. A number of studies have taken physiological assessment of the heat shock response (HSR) into the intertidal both as a tool for examining the HSR in nature and for examining the utility of HSR molecules as population or community level indicators. Most commonly, immunodetection of the total pool of the Hsp70 family of isoforms is used. Here we present data on levels of Hsp70 in intertidal organisms from microhabitat to the mesoscale. Our data and previously published work show that Hsp70 levels vary at all scales examined, similar to other physical and biological variables of interest. This demonstrates both the potential utility of Hsp70 detection as a molecular tool for field biologists and to the care that must be taken in assessing scale of variation when looking for potential bioindicator molecules.
将生物体对热应激的反应与群落结构模式联系起来的一个重要步骤是确定可察觉的变异水平在什么尺度上表现出来。生物体可能做出反应的温度信号在许多空间尺度上发生变化，包括微生境、潮高、地点和纬度。一些研究已经将热激反应 (HSR) 的生理评估带入潮间带，既是为了研究自然界中的 HSR，也是为了研究 HSR 分子作为种群或群落水平指标的效用。最常用的方法是免疫检测 Hsp70 家族同工型的总池。在这里，我们展示了从微生境到中尺度的潮间带生物体内 Hsp70 的水平数据。我们的数据和以前发表的工作表明，Hsp70 水平在所有研究的尺度上都发生变化，与其他感兴趣的物理和生物变量相似。这既证明了 Hsp70 检测作为野外生物学家的分子工具的潜在效用，也证明了在寻找潜在的生物指标分子时，必须注意评估变化的尺度。
