Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal.
In Vivo. 2011 Jul-Aug;25(4):625-31.
A serological survey was conducted in Macao, China, in 753 individuals, with the objective of looking for antibodies to the mosquito, Aedes albopictus (Skuse 1894) (Diptera: Culicidae), and to dengue, before the occurrence of any autochthonous dengue cases. Blood samples were collected at several public health services, a questionnaire was answered, and enzyme-linked immunosorbant assay (ELISA) and Western blot techniques were performed with extracts of mosquito head and thorax (HT). Anti-Aedes albopictus IgG antibodies were present in titres 1:10(2)-1:10(3) in 9%, and in titres 1:10(4)-1:10(5) in 42% of the sera tested. This reactivity was more frequent (59%) in the population which had resided only in Macao in the 2 years previous to the survey, as opposed to those that had also resided in other areas (50%). From the 230 reactive sera selected for immunoblot, 48 (21%) reacted with a wide range of proteins from above 224 kDa to 21 kDa, with different patterns according to individual sera. Proteins in the intervals 35.3-28.7 kDa and 28.7-21.1 kDa were labelled by the greatest number of sera, 15 and 19 respectively. The presence of anti-Aedes albopictus antibodies presented a statistical relation to skin reaction to mosquito bites, but immunoblot patterns did not. Anti-dengue IgG antibodies were found in 48% of the subjects, with a higher proportion in people who had resided out of Macao, or who were nationals from dengue-endemic neighboring countries. Anti-dengue reactivity was in agreement with anti-mosquito reactivity in half of the population. It would be interesting to see if this proportion has changed since dengue became endemic in Macao in 2001.
在中国澳门，对 753 人进行了血清学调查，目的是在出现任何本地登革热病例之前寻找针对白纹伊蚊（Skuse 1894）（双翅目：库蚊科）的抗体和登革热抗体。在几个公共卫生服务机构采集血样，回答问卷，并使用蚊头和胸（HT）提取物进行酶联免疫吸附试验（ELISA）和 Western blot 技术。在检测的血清中，9%的血清抗白纹伊蚊 IgG 抗体的效价为 1:10(2)-1:10(3)，42%的血清抗白纹伊蚊 IgG 抗体的效价为 1:10(4)-1:10(5)。与仅在调查前 2 年居住在澳门的人群（59%）相比，曾居住在其他地区的人群（50%）的这种反应性更为频繁。从 230 份选择用于免疫印迹的反应性血清中，有 48 份（21%）与来自 224 kDa 以上至 21 kDa 的多种蛋白发生广泛反应，根据个体血清的不同而呈现不同的模式。在 35.3-28.7 kDa 和 28.7-21.1 kDa 之间的间隔内的蛋白被数量最多的血清标记，分别为 15 和 19。抗白纹伊蚊抗体的存在与蚊虫叮咬后的皮肤反应呈统计学关系，但免疫印迹模式则没有。48%的研究对象存在抗登革热 IgG 抗体，在居住在澳门以外地区或来自登革热流行邻国的人群中，这一比例更高。在一半的人群中，抗登革热反应与抗蚊反应一致。自 2001 年澳门登革热流行以来，看看这一比例是否发生了变化会很有趣。
