Lin Biaoyang, Wang Jun, Cheng Yin
The Institute for Systems Biology, Seattle, WA, 98021 USA.
Recent Pat Biomed Eng. 2008;2008(1):60-67. doi: 10.2174/1874764710801010060.
We are now witnessing a new genomic revolution due to the arrival and continued advancements in the next-generation high-throughput sequencing technologies, which encompass sequencing by synthesis including fluorescent in situ sequencing (FISSEQ) and pyrosequencing, sequencing by ligation including using polony amplification and supported oligonucleotide detection (SOLiD), sequencing by hybridization in combination with sequencing-by-ligation and nanopore technology, nanopore sequencing and other novel sequencing technologies using nano-transistor array, scanning tunneling microscopy and nanowire molecule sensors etc. We review here major technologies and recent patents for achieving high-throughput, ultra-fast, extremely cheap, and highly accurate sequencing. We will see enormous impacts of these next-generation sequencing methods for solving complex biological problems and for ushering in the practice of personalized medicine.
由于新一代高通量测序技术的出现及其持续发展，我们正在见证一场新的基因组革命。这些技术包括基于合成的测序，如荧光原位测序（FISSEQ）和焦磷酸测序；基于连接的测序，如使用克隆扩增和支持寡核苷酸检测（SOLiD）；结合连接测序和纳米孔技术的杂交测序、纳米孔测序，以及其他使用纳米晶体管阵列、扫描隧道显微镜和纳米线分子传感器等的新型测序技术。我们在此回顾实现高通量、超快速、极其廉价和高精度测序的主要技术及近期专利。我们将看到这些新一代测序方法对解决复杂生物学问题和推动个性化医疗实践产生的巨大影响。
