HPV 阳性和 HPV 阴性口腔鳞状细胞癌中朗格汉斯细胞的免疫组织化学染色。
Immunohistochemical staining of Langerhans cells in HPV-positive and HPV-negative cases of oral squamous cells carcinoma.
机构信息
School of Dentistry, Federal University of Ceará, Sobral, CE, Brazil.
出版信息
J Appl Oral Sci. 2011 Aug;19(4):378-83. doi: 10.1590/s1678-77572011005000013. Epub 2011 Jun 24.
UNLABELLED
The Human Papillomavirus (HPV) has been strongly implicated in development of some cases of oral squamous cell carcinoma (OSCC). However, the immunological system somehow reacts against the presence of this virus. Among the cells involved in such mechanism of defense Langerhans cells (LC) stand out, which are responsible for processing and presenting antigens.
OBJECTIVES
The purposes of this study were to investigate the presence of HPV DNA and to evaluate the immunohistochemical reactivity for Langerhans cells between HPV-positive and HPV-negative OSCC. Twenty-seven cases of OSSC were evaluated.
MATERIAL AND METHODS
DNA was extracted from paraffin-embedded tissue samples and amplified by Polymerase Chain Reaction (PCR) for the detection of HPV DNA. Viral typing was performed by dot blot hybridization. Immunohistochemistry was performed by the Streptavidin-biotin technique.
RESULTS
From the 27 cases, 9 (33.3%) were HPV-positive and 18 (66.0%) HPV-negative. HPV 18 was the most prevalent viral type (100% cases) and infection with HPV-16 (co-infection) was detected in only 1 case. In the OSCC specimens examined, immunoreactivity to S-100 antibody was detected in all cases, with a mean number of 49.48±30.89 Langerhans cells positive for immunostaining. The mean number of immunostained Langerhans cells was smaller in the HPV-positive cases (38 cells/case) than in the HPV-negative cases (42.5 cells/case), but this difference was not significant (p=0.38).
CONCLUSIONS
The low frequency of detection of HPV DNA in OSCC indicates a possible participation of the virus in the development and progression of only a subgroup of these tumors. There was no association between the immunohistochemical labeling for Langerhans cells (S-100+) and HPV infection of in OSSC. These findings suggest that the presence of HPV in such OSCC cases could not alter the immunological system, particularly the Langerhans cells.
未加标签
人乳头瘤病毒（HPV）已被强烈暗示与某些口腔鳞状细胞癌（OSCC）病例的发展有关。然而，免疫系统以某种方式对这种病毒的存在作出反应。在参与这种防御机制的细胞中，朗格汉斯细胞（LC）尤为突出，它们负责处理和呈递抗原。
目的
本研究的目的是检测 HPV DNA 的存在，并评估 HPV 阳性和 HPV 阴性 OSCC 之间朗格汉斯细胞的免疫组织化学反应。评估了 27 例 OSCC 病例。
材料和方法
从石蜡包埋组织样本中提取 DNA，并通过聚合酶链反应（PCR）扩增以检测 HPV DNA。通过斑点杂交进行病毒分型。通过链霉亲和素-生物素技术进行免疫组织化学。
结果
在 27 例病例中，9 例（33.3%）为 HPV 阳性，18 例（66.0%）为 HPV 阴性。HPV 18 是最常见的病毒类型（100%的病例），仅在 1 例中检测到 HPV-16（共同感染）。在检查的 OSCC 标本中，所有病例均检测到 S-100 抗体免疫反应，免疫染色阳性的朗格汉斯细胞平均数量为 49.48±30.89 个。HPV 阳性病例的免疫染色朗格汉斯细胞平均数量（38 个/例）小于 HPV 阴性病例（42.5 个/例），但差异无统计学意义（p=0.38）。
结论
OSCC 中 HPV DNA 的低检出频率表明病毒可能仅参与了这些肿瘤亚组的发生和发展。在 OSCC 中，朗格汉斯细胞（S-100+）的免疫组织化学标记与 HPV 感染之间没有关联。这些发现表明，HPV 在这些 OSCC 病例中的存在不能改变免疫系统，特别是朗格汉斯细胞。
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