Liu Lijuan, Jiang Wenhong, Zhang Lijie, Li Fang, Zhang Qingbo
Key Laboratory of Chemical Engineering Process and Technology for High-Efficiency Conversion, College of Heilongjiang Province, Harbin, P. R. China; Department of Pharmaceutical Engineering, Heilongjiang University, Harbin, P. R. China.
J Sep Sci. 2011 Aug;34(15):1834-44. doi: 10.1002/jssc.201000851. Epub 2011 Jun 27.
Shuanghuanglian injection, a famous composite formula in China, is a combination of Flos lonicerae japonicae, Radix scutellariae and Fructus forsythiae. To develop a method to overall evaluate the correlation between the formula and its three raw herbs, the representative LC fingerprint of Shuanghuanglian injection was established from 12 batches of samples. Ten peaks were selected as the common peaks to evaluate the similarities of different samples from different manufacturers. Hierarchical clustering analysis was used as a powerful pattern recognition tool to identify and classify Shuanghuanglian injection from different factories. In addition, chemical correlation between the formula and the three raw herbs was evaluated qualitatively through comparison of their chromatograms under the same HPLC conditions. The results showed that peak 1, 2, 3 and 9 in the formula fingerprint were characteristic components of Flos lonicerae japonicae, peaks 5, 6 and 8 were that of F. forsythiae, peak 10 was that of R. scutellariae, while peak 7 was the common component of Flos lonicerae japonicae and F. forsythiae, peak 4 was addressed to F. forsythiae and it was also the product of the manufacturing procedure.
双黄连注射液是中国著名的复方制剂，由金银花、黄芩和连翘组成。为建立一种全面评价该复方制剂与其三味原料药之间相关性的方法，从12批样品中建立了双黄连注射液的代表性液相色谱指纹图谱。选取10个峰作为共有峰，以评价不同厂家不同样品的相似度。层次聚类分析作为一种强大的模式识别工具，用于鉴别和分类不同厂家的双黄连注射液。此外，通过比较相同高效液相色谱条件下它们的色谱图，对该复方制剂与三味原料药之间的化学相关性进行了定性评价。结果表明，复方指纹图谱中的峰1、2、3和9为金银花的特征成分，峰5、6和8为连翘的特征成分，峰10为黄芩的特征成分，而峰7为金银花和连翘的共有成分，峰4归属于连翘且也是生产过程的产物。
