Dong Yang, Ji Guang, Cao Aili, Shi Jianrong, Shi Hailian, Xie Jianqun, Wu Dazheng
Institute of Chinese Materia Medica, Shanghai Key Laboratory of Complex Prescription, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhongguo Zhong Yao Za Zhi. 2011 Mar;36(6):790-4.
To study the effects and mechanisms of sinensetin on proliferation and apoptosis of human AGS gastric cancer cells.
MTT assay was used to detect the growth inhibition rates of human AGS gastric cancer cells treated with sinsesectin in different concentrations and times. The cell cycle distribution was measured by flow cytometry. The apoptosis was examined by Annexin-FITC/PI staining and DNA fragment analysis. The apoptosis morphology was observed by inverted fluorescence microscope after Hoechst 33342 staining. The protein expressions of p21 and p53 were detected by western blot.
MTT assay showed that sinensetin inhibited the growth of AGS gastric cancer cells in a dose- and time-dependent manner. Sinensetin blocked AGS cells in G2/ M and increased the apoptosis rates of AGS cells in a dose-dependent manner. DNA ladder was observed in cells treated with 60 micromol x L(-1) sinensetin for 48 h. The typical apoptotic morphological changes including cell nucleus shrinkage, chromatin condensation and apoptotic bodies were observed when treated with different dose of sinensetin. Western blot showed that sinensetin increased expressions of p53 and p21 in a dose-dependent manner.
Sinensetin could inhibit human AGS gastric cancer cells proliferation and induce cell cycle block in G2/M phase and apoptosis. The up regulation of p53 and p21 protein might be one of the mechanisms.
研究橙皮素对人AGS胃癌细胞增殖和凋亡的影响及其机制。
采用MTT法检测不同浓度和时间的橙皮素处理后人AGS胃癌细胞的生长抑制率。通过流式细胞术测定细胞周期分布。采用Annexin-FITC/PI染色和DNA片段分析检测细胞凋亡。经Hoechst 33342染色后，用倒置荧光显微镜观察凋亡形态。通过蛋白质免疫印迹法检测p21和p53的蛋白表达。
MTT法显示橙皮素以剂量和时间依赖性方式抑制AGS胃癌细胞的生长。橙皮素使AGS细胞阻滞于G2/M期，并以剂量依赖性方式增加AGS细胞的凋亡率。用60 μmol·L⁻¹橙皮素处理细胞48小时后观察到DNA梯状条带。用不同剂量橙皮素处理后，观察到典型的凋亡形态学变化，包括细胞核缩小、染色质浓缩和凋亡小体。蛋白质免疫印迹法显示橙皮素以剂量依赖性方式增加p53和p21的表达。
橙皮素可抑制人AGS胃癌细胞增殖，诱导细胞周期阻滞于G2/M期并诱导凋亡。p53和p21蛋白的上调可能是其机制之一。
