College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.
Immunopharmacol Immunotoxicol. 2012 Apr;34(2):191-5. doi: 10.3109/08923973.2011.590499. Epub 2011 Jun 28.
Polygonum cuspidatum (PC) has been used for the treatment of arthritis and urinary diseases in traditional medicine. Despite recent evidence that PC has anti-oxidant, anti-tumoral, and anti-inflammatory effects, analgesic and anti-inflammatory effects of PC have not been elucidated yet in vivo. Thus, in the present study, analgesic and anti-inflammatory effects of ethyl acetate extract of PC (EAPC) were investigated in vivo for the first time. Hot plate test and tail-flick test revealed that EAPC at 200 mg/kg exerts analgesic effect (p < 0.05). In contrast, EAPC did not show significant analgesic effect in acetic acid-induced writhing test. Serotonin-induced paw edema model and Freund's complete adjuvant (FCA)-induced adjuvant arthritis model were used to examine anti-inflammatory effect of EAPC in vivo. In serotonin-induced paw edema model, EAPC suppressed swelling inflammatory response within 12 min after serotonin injection, at both 100- and 200-mg/kg dose (p < 0.05). Consistently, in FCA-induced adjuvant arthritis model, FCA at 200 mg/kg significantly suppressed FCA-induced joint swelling within 3 days (p < 0.05), whereas FCA at 100 mg/kg showed the similar result within 5 days (p < 0.05). Furthermore, EAPC effectively inhibited positive responses of c-reactive protein and rheumatoid factor compared to untreated control. Taken together, our findings suggest that EAPC can be a potent candidate for rheumatoid arthritis treatment.
虎杖在传统医学中被用于治疗关节炎和泌尿系统疾病。尽管最近有证据表明虎杖具有抗氧化、抗肿瘤和抗炎作用，但虎杖在体内的镇痛和抗炎作用尚未阐明。因此，本研究首次在体内研究了虎杖乙酸乙酯提取物（EAPC）的镇痛和抗炎作用。热板试验和甩尾试验表明，EAPC 200mg/kg 具有镇痛作用（p<0.05）。然而，EAPC 在醋酸诱导的扭体试验中没有表现出明显的镇痛作用。使用 5-羟色胺诱导的爪肿胀模型和完全弗氏佐剂（FCA）诱导的佐剂性关节炎模型来研究 EAPC 在体内的抗炎作用。在 5-羟色胺诱导的爪肿胀模型中，EAPC 在 5-羟色胺注射后 12 分钟内抑制肿胀炎症反应，在 100 和 200mg/kg 剂量下均有抑制作用（p<0.05）。同样，在 FCA 诱导的佐剂性关节炎模型中，FCA 200mg/kg 在 3 天内显著抑制 FCA 诱导的关节肿胀（p<0.05），而 FCA 100mg/kg 在 5 天内表现出相似的结果（p<0.05）。此外，与未处理的对照组相比，EAPC 能有效抑制 C 反应蛋白和类风湿因子的阳性反应。综上所述，我们的研究结果表明，EAPC 可能是治疗类风湿关节炎的有效候选药物。
