Qilu Hospital, Shandong University, Jinan, P.R. China.
Lipids Health Dis. 2011 Jun 29;10:108. doi: 10.1186/1476-511X-10-108.
The purpose of this study was to identify the affect on the proliferation Eca-109 cells treated with oxidized low-density lipoprotein (ox-LDL) combined with adriamycin (ADM).
Eca-109 cell were cultured in the presence of oxLDL/ADM, and cell proliferation tested by MTT and cell apoptosis was monitored by the proportion of apoptosis and cell cycle by flow cytomester. We simultaneously evaluated the level of associated- apoptosis Bcl-2, Bax, and Caspase-3 gene mRNA and protein.
OxLDL were cytotoxic and activate apoptosis. OxLDL combined with ADM significant enhanced the proportion rate of apoptosis on a time and dose dependency. The expressions of the inhibiting apoptosis Bcl-2 gene mRNA and protein were down regulated, whereas, the expressions of the promoting apoptosis Bax, and Caspase-3 genes mRNA and protein were up regulation.
These results suggested that oxLDL have cytotoxicity and activate apoptosis on the Eca-109 cells. OxLDL combined with ADM have a synergistic effect on the apoptosis induced Eca-109 cells. Furthermore, oxLDL may contribute to the improvement of clinical chemotherapy of cancer need to make further investigation.
本研究旨在探讨氧化型低密度脂蛋白（ox-LDL）与阿霉素（ADM）联合作用对食管癌细胞（Eca-109）增殖的影响。
将 Eca-109 细胞分别在 oxLDL/ADM 存在的条件下进行培养，通过 MTT 法检测细胞增殖情况，采用流式细胞术检测细胞凋亡比例和细胞周期，同时检测相关凋亡基因 Bcl-2、Bax 和 Caspase-3 的 mRNA 和蛋白表达水平。
ox-LDL 具有细胞毒性，并能诱导细胞凋亡。ox-LDL 联合 ADM 呈时间和剂量依赖性显著增强细胞凋亡的比例。抑制凋亡基因 Bcl-2 的 mRNA 和蛋白表达下调，而促进凋亡基因 Bax 和 Caspase-3 的 mRNA 和蛋白表达上调。
这些结果表明 ox-LDL 对 Eca-109 细胞具有细胞毒性和促凋亡作用，ox-LDL 联合 ADM 对诱导 Eca-109 细胞凋亡具有协同作用。ox-LDL 可能有助于改善癌症的临床化疗效果，需要进一步研究。
