Department of Otorhinolaryngology, Head and Neck Surgery, University of Munich, Munich, Germany.
Am J Rhinol Allergy. 2011 Jan-Feb;25(1):36-9. doi: 10.2500/ajra.2011.25.3551.
The aim of this study was to investigate recombinant marker allergens in patients with allergic rhinoconjunctivitis to tree and grass pollens.
Sera of 260 tree pollen- and 282 grass pollen-allergic patients were analyzed. Bet v 1 and Phl p 1/p 5 were used as marker allergens for allergies to pollen from birch and grasses. Profilin (Bet v 2 and Phl p 12) and calcium-binding protein (Bet v 4 and Phl p 7) were used as markers for polysensitization.
Two hundred thirty-nine (92%) patients tested positive for IgE anti-Bet v 1. All of them were positive for IgE antibodies against natural birch extract (t3), hazel (t4), and alder (t2). Bet v 1 in combination with t3 identified allergies from related trees with a sensitivity of 99.2%. Two hundred fifty-six patients (91%) tested positive for IgE anti-Phl p 1 and/or anti-Phl p 5. All of them were positive for IgE antibodies against natural timothy (g6) and rye (g12) extract. Phl p 1/p 5 and natural timothy extract identified allergies from grasses and rye with a sensitivity of 99.3%. All Patients reacting to the cross-reactive allergens Bet v 2, Phl p 12, Bet v 4, and Phl p 7 were polysensitized.
Bet v 1 in combination with natural birch pollen extract identifies allergies from related trees with a sensitivity of 99.2%. Phl p 1, Phl p 5, and natural timothy extract identifies allergies from grasses and rye in 99.3%. Reactivity to the allergens Bet v 2, Phl p 12, Bet v 4, and Phl p 7 identifies polysensitization and cross-reactivity.
本研究旨在探讨花粉过敏症患者的树和草花粉过敏的重组标记过敏原。
分析了 260 例树花粉过敏和 282 例草花粉过敏患者的血清。Bet v 1 和 Phl p 1/p 5 被用作桦树和草花粉过敏的标记过敏原。丝氨酸蛋白酶抑制剂（Bet v 2 和 Phl p 12）和钙结合蛋白（Bet v 4 和 Phl p 7）被用作多敏化的标志物。
239 例（92%）患者 IgE 抗 Bet v 1 阳性。他们均对天然桦树提取物（t3）、榛子（t4）和桤木（t2）呈 IgE 抗体阳性。Bet v 1 联合 t3 可鉴定相关树木过敏，灵敏度为 99.2%。256 例（91%）患者 IgE 抗 Phl p 1 和/或抗 Phl p 5 阳性。他们均对天然梯牧草（g6）和黑麦（g12）提取物呈 IgE 抗体阳性。Phl p 1/p 5 和天然梯牧草提取物可鉴定草和黑麦过敏，灵敏度为 99.3%。所有对交叉反应过敏原 Bet v 2、Phl p 12、Bet v 4 和 Phl p 7 有反应的患者均为多敏化。
Bet v 1 联合天然桦树花粉提取物可鉴定相关树木过敏，灵敏度为 99.2%。Phl p 1、Phl p 5 和天然梯牧草提取物可鉴定草和黑麦过敏，灵敏度为 99.3%。对过敏原 Bet v 2、Phl p 12、Bet v 4 和 Phl p 7 的反应可鉴定多敏化和交叉反应。
