Kumar S, Boyle D B
Commonwealth Scientific Industrial Research Organisation, Australia Animal Health Laboratory, Geelong, Victoria.
Virology. 1990 Nov;179(1):151-8. doi: 10.1016/0042-6822(90)90284-x.
A novel bidirectional promoter element of fowlpox virus (FPV) was characterized by transcription analysis, transient expression assays, and recombinant virus construction. This promoter element contained an early/late and a late function in opposite orientation, all within 42 bp of the DNA sequence. The 42-bp sequence was sufficient to express two reporter genes simultaneously in a temporally regulated manner. Both early and late mRNA from the early/late promoter originated at the same TAAAT motif and lacked a long 5'poly(A) leader sequence. Late mRNA, initiated from a TAAAT motif of the oppositely oriented late promoter strand, had a leader sequence of approximately 26 bases. Sequence alignment of two strands of the bidirectional element showed that 28 of 42 bases matched. Because of its small and defined size as well as unique structure, this bidirectional promoter should prove to be an important tool in defining the sequences required for the temporal regulation of poxvirus genes.
通过转录分析、瞬时表达测定和重组病毒构建，对禽痘病毒（FPV）一种新型双向启动子元件进行了表征。该启动子元件在DNA序列的42 bp范围内包含一个早期/晚期和一个晚期功能，二者方向相反。42 bp的序列足以以时间调控的方式同时表达两个报告基因。来自早期/晚期启动子的早期和晚期mRNA均起始于相同的TAAAT基序，且缺乏长的5'聚腺苷酸前导序列。从反向晚期启动子链的TAAAT基序起始的晚期mRNA具有约26个碱基的前导序列。双向元件两条链的序列比对显示，42个碱基中有28个匹配。由于其大小明确且结构独特，该双向启动子应被证明是确定痘病毒基因时间调控所需序列的重要工具。
