Heine H G, Boyle D B
CSIRO, Australian Animal Health Laboratory, Geelong, Victoria.
Arch Virol. 1993;131(3-4):277-92. doi: 10.1007/BF01378632.
Two fowlpox virus recombinants were constructed which expressed the host-protective antigen, VP2, of infectious bursal disease virus (IBDV). Recombinant FPV-VP 2.4.3 contained the gene for the VP 2-VP4-VP3 polyprotein under the control of the vaccinia virus late promoter P.L 11 inserted within the thymidine kinase (TK) gene of FPV. In infected chicken embryo skin (CES) cells VP2 and VP3 proteins were correctly processed from the polyprotein precursor molecule. Recombinant FPV-VP2 contained only the VP2 encoding region under the control of the fowlpox early/late promoter P.E/L inserted immediately downstream of the TK gene. The expression level of VP2 from FPV-VP2 was approximately 5 times higher than from FPV-VP2.4.3. Wing web inoculation of birds resulted in the development of typical fowlpox lesions and the development of antibodies to FPV with either of the recombinants, but only birds vaccinated with FPV-VP2 developed antibodies to IBDV. When challenged with IBDV (strain 002-73), a significant level of protection was provided by FPV-VP2 vaccination, although the level was lower than the protection provided by an oil adjuvanted inactivated whole IBDV vaccine. Birds vaccinated with FPV-VP2.4.3 were not protected from infection as assessed by ELISA for the presence of IBD virus in bursae.
构建了两种禽痘病毒重组体，它们表达传染性法氏囊病病毒（IBDV）的宿主保护性抗原VP2。重组禽痘病毒FPV-VP 2.4.3含有VP2-VP4-VP3多聚蛋白基因，该基因由痘苗病毒晚期启动子P.L 11控制，插入到禽痘病毒的胸苷激酶（TK）基因内。在感染的鸡胚皮肤（CES）细胞中，VP2和VP3蛋白从多聚蛋白前体分子中正确加工而来。重组禽痘病毒FPV-VP2仅包含VP2编码区，由禽痘病毒早期/晚期启动子P.E/L控制，紧接在TK基因下游插入。FPV-VP2中VP2的表达水平比FPV-VP2.4.3高约5倍。用这两种重组体之一对鸡翼蹼接种后，会出现典型的禽痘病变并产生针对禽痘病毒的抗体，但只有接种FPV-VP2的鸡产生了针对IBDV的抗体。当用IBDV（002-73株）攻击时，FPV-VP2疫苗接种提供了显著水平的保护，尽管该水平低于油佐剂灭活全IBDV疫苗提供的保护。通过ELISA检测法氏囊中是否存在IBD病毒评估，接种FPV-VP2.4.3的鸡未受到感染保护。
