A full-length murine 2-5A synthetase cDNA transfected in NIH-3T3 cells impairs EMCV but not VSV replication.

作者信息

Coccia E M, Romeo G, Nissim A, Marziali G, Albertini R, Affabris E, Battistini A, Fiorucci G, Orsatti R, Rossi G B

机构信息

Laboratorio di Virologia, Istituto Superiore di Sanità, Rome, Italy.

出版信息

Virology. 1990 Nov;179(1):228-33. doi: 10.1016/0042-6822(90)90292-y.

Treatment of cells with interferons (IFNs) induces resistance to virus infection. The 2'-5'oligo A (2-5A) synthetase/RNase L is one of the pathways leading to translation inhibition induced by IFN treatment. A murine cDNA encoding the 43-kDa 2-5A synthetase was cloned and sequenced. NIH-3T3 cell clones transfected with this cDNA expressed the enzymatic activity to various extents and exhibited resistance to encephalomyocarditis virus (EMCV) but not to vesicular stomatitis virus replication. The specific resistance to EMCV can be attributed to 2-5A synthetase.