Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares (ICETA), Universidade do Porto, 4485-661 Vairão, Portugal.
J Biomed Mater Res B Appl Biomater. 2011 Oct;99(1):170-9. doi: 10.1002/jbm.b.31884. Epub 2011 Jun 28.
A glass-reinforced hydroxyapatite (HA) composite, recently registered as Bonelike®, was developed for bone grafting. This biomaterial is composed of a modified HA matrix with α- and β-tricalcium phosphate secondary phases and ionic species that mimic the chemical composition of human bone. Several in vitro and in vivo studies have confirmed the benefits of these properties. However, these studies were all executed with Bonelike® polygonal granules obtained by crushing. In this study, Bonelike® pellets were produced through a patented process, which required the use of techniques such as extrusion and spheronization. The final product presented a homogeneous size, a 55.1% global porosity and a spherical shape. This spherical shape permitted a better adaptation to the implantation site and improved injectability. Additionally, it also may contribute to formation of macropores as pellets packaging leaves open spaces. After implantation of Bonelike® polygonal granules and Bonelike® pellets in monocortical defects in sheep for 8 and 12 weeks, light microscopy and scanning electron microscopy showed extensive osteointegration simultaneously with bone regeneration for both presentations. Histomorphometric analysis did not reveal statistically significant differences between defects treated with Bonelike® polygonal granules and Bonelike® pellets, which suggests similar in vivo performances.
一种玻璃增强的羟基磷灰石（HA）复合材料，最近被注册为 Bonelike®，是为骨移植而开发的。这种生物材料由经过改良的 HA 基质与 α-和 β-磷酸三钙的次级相和模拟人骨化学成分的离子种类组成。几项体外和体内研究证实了这些特性的益处。然而，这些研究都是用 Bonelike®多边形颗粒进行的，这些颗粒是通过粉碎获得的。在这项研究中，通过专利工艺生产了 Bonelike®颗粒，该工艺需要使用挤出和造粒等技术。最终产品呈现均匀的尺寸、55.1%的总孔隙率和球形。这种球形形状允许更好地适应植入部位并提高可注射性。此外，由于颗粒包装留下的空隙，它还可能有助于形成大孔。将 Bonelike®多边形颗粒和 Bonelike®颗粒植入绵羊的单皮质缺损中 8 周和 12 周后，光镜和扫描电子显微镜显示两种情况下都有广泛的骨整合和骨再生。组织形态计量学分析显示，用 Bonelike®多边形颗粒和 Bonelike®颗粒治疗的缺陷之间没有统计学上的显著差异，这表明它们在体内的性能相似。
