Department of Veterinary Resources, Weizmann Institute of Science, Rehovot, 76100, Israel.
J Biophotonics. 2011 Sep;4(9):645-9. doi: 10.1002/jbio.201100033. Epub 2011 Jun 28.
We present a multi-modal optical diagnostic approach utilizing a combined use of Fluorescence Intravital Microscopy (FIM), Dynamic Light Scattering (DLS) and Spectrally Enhanced Microscopy (SEM) modalities for in vivo imaging of tumor vascular network and blood microcirculation. FIM is used for imaging of tumor surroundings and microenvironment, SEM provides information regarding blood vessels topography, whereas DLS is applied for functional imaging of vascular network and blood microcirculation. This complementary combination of the imaging approaches is extremely useful for functional in vivo imaging of blood vasculature and tumor microenvironment. The technique has also a great potential in vascular biology and can significantly expand the capabilities of tumor angiogenesis studies and notably contribute to the development of cancer treatment.
我们提出了一种多模态光学诊断方法，该方法结合使用荧光活体显微镜（FIM）、动态光散射（DLS）和光谱增强显微镜（SEM）模式，用于肿瘤血管网络和血液微循环的体内成像。FIM 用于肿瘤周围环境和微环境的成像，SEM 提供有关血管拓扑结构的信息，而 DLS 则用于血管网络和血液微循环的功能成像。这些成像方法的互补组合对于血液血管和肿瘤微环境的功能体内成像非常有用。该技术在血管生物学方面也具有巨大的潜力，可以显著扩展肿瘤血管生成研究的能力，并为癌症治疗的发展做出重要贡献。
