用于监测中风小鼠模型血流动力学变化的快速同步双波长激光散斑成像系统。
Fast synchronized dual-wavelength laser speckle imaging system for monitoring hemodynamic changes in a stroke mouse model.
机构信息
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
出版信息
Opt Lett. 2012 Oct 1;37(19):4005-7. doi: 10.1364/OL.37.004005.
Abstract
In this Letter, we describe a newly developed synchronized dual-wavelength laser speckle contrast imaging system, which contains two cameras that are synchronously triggered to acquire data. The system can acquire data at a high spatiotemporal resolution (up to 500 Hz for ~1000×1000 pixels). A mouse model of stroke is used to demonstrate the capability for imaging the fast changes (within tens of milliseconds) in oxygenated and deoxygenated hemoglobin concentration, and the relative changes in blood flow in the mouse brain, through an intact cranium. This novel imaging technology will enable the study of fast hemodynamics and metabolic changes in vascular diseases.
摘要
在这封信件中，我们描述了一种新开发的同步双波长激光散斑对比成像系统，该系统包含两个同步触发以获取数据的相机。该系统可以以高时空分辨率（高达 500 Hz，用于~1000×1000 像素）获取数据。我们使用中风的小鼠模型演示了通过完整颅骨成像来检测氧合和去氧血红蛋白浓度以及小鼠大脑血流的相对变化的快速变化（在数十毫秒内）的能力。这项新型成像技术将使研究血管疾病中的快速血液动力学和代谢变化成为可能。
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