Ma Chao-Qun, Chen Guo-Qing, Wei Bai-Lin, Shi Yuan-Ping, Gu Ling, Gao Shu-Mei, Zhu Tuo
School of Science, Jiangnan University, Wuxi 214122, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2011 Apr;31(4):1065-8.
The fluorescence excitation spectra and absorption spectra of six kinds of erythrosine solutions with concentrations of 10, 20, 30, 40, 50 and 60 microg x mL(-1) were experimentally measured. It was found that the fluorescence excitation peaks are both located at 530 nm significantly when the concentrations of erythrosine solutions are 10 and 20 microg x mL(-1). However, the linetype saltation of fluorescence excitation spectrum occurs as the concentration of erythrosine solution is above 30 microg x mL(-1). The valley is located at 530 nm and two new peaks appear at both flanks of the valley. Compared with fluorescence excitation spectra, the absorption spectra of erythrosine solutions are without saltation and the peaks are all located at 530 nm. According to calculations and a series of contrast experiments, it was demonstrated that the absorption characteristic of erythrosine and the spectral measurement mode conspire to cause the saltation of fluorescence excitation spectra. The results can provide guidance for further research on physical and chemical properties of erythrosine, and offer help and reference for study on saltation behavior in fluorescence excitation spectra and improvement in spectral measuring mode.
实验测量了浓度分别为10、20、30、40、50和60μg·mL⁻¹的六种赤藓红溶液的荧光激发光谱和吸收光谱。结果发现，当赤藓红溶液浓度为10和20μg·mL⁻¹时，荧光激发峰均显著位于530nm处。然而，当赤藓红溶液浓度高于30μg·mL⁻¹时，荧光激发光谱出现线型突变。谷值位于530nm处，且在谷值两侧出现两个新峰。与荧光激发光谱相比，赤藓红溶液的吸收光谱无突变，峰均位于530nm处。通过计算和一系列对比实验表明，赤藓红的吸收特性和光谱测量模式共同导致了荧光激发光谱的突变。该结果可为进一步研究赤藓红的物理化学性质提供指导，为研究荧光激发光谱中的突变行为及改进光谱测量模式提供帮助和参考。
