Institute of Biosciences, Botany Department, University of São Paulo, São Paulo, SP, Brazil.
J Sci Food Agric. 2011 Oct;91(13):2363-70. doi: 10.1002/jsfa.4468. Epub 2011 May 17.
Chloroform, ethyl acetate and methanol extracts of a sample of red propolis from the state of Alagoas (northeast Brazil) were analyzed by gas chromatography-mass spectrometry and high-performance liquid chromatography-diode array detection-electrospray ionization-mass spectrometry. Antimicrobial and antioxidant activities were also obtained.
The propolis sample contained low content of narigenin-8-C-hexoside, this being the first report of a C-glycoside in propolis. The main constituent found was characterized as 3,4,2',3'-tetrahydroxychalcone. Other important constituents were the chalcone isoliquiritigenin, the isoflavans (3S)-vestitol, (3S)-7-O-methylvestitol, the pterocarpan medicarpin, the phenylpropenes trans-anethol, methyl eugenol, elimicin, methoxyeugenol and cis-asarone, and the triterpenic alcohols lupeol and α- and β- amyrins. The methanol extract exhibited high antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl and β-carotene/linoleic acid assay methods, and antimicrobial activity toward Gram-positive and Gram-negative bacteria.
Structures are suggested for new substances never before seen in any kind of propolis. This is the first report of 3,4,2',3'-tetrahydroxychalcone and a flavone C-glycoside in a propolis sample.
从巴西东北部阿拉戈斯州的一种红色蜂胶样品中提取的氯仿、乙酸乙酯和甲醇提取物，通过气相色谱-质谱联用仪和高效液相色谱-二极管阵列检测-电喷雾电离-质谱联用仪进行了分析。还获得了抗菌和抗氧化活性。
该蜂胶样品中含有低含量的芹菜素-8-C-己糖苷，这是蜂胶中首次报道 C-糖苷。发现的主要成分被鉴定为 3,4,2',3'-四羟基查尔酮。其他重要成分包括查尔酮异甘草素、异黄酮（3S）-维斯托醇、（3S）-7-O-甲基维斯托醇、紫檀烷 medicarpin、苯丙烯反茴香醇、甲基丁香酚、乙氧甲酚、甲氧基丁香酚和顺式细辛脑，以及三萜醇芦醇和α-和β-桉油醇。甲醇提取物通过 2,2-二苯基-1-苦基肼和β-胡萝卜素/亚油酸测定方法表现出高抗氧化活性，并且对革兰氏阳性和革兰氏阴性细菌具有抗菌活性。
提出了从未在任何类型的蜂胶中见过的新物质的结构。这是首次在蜂胶样品中报道 3,4,2',3'-四羟基查尔酮和黄酮 C-糖苷。
