细胞色素氧化酶在有机溶剂中的光谱和催化特性

Spectral and catalytic properties of cytochrome oxidase in organic solvents.

作者信息

Bona M, Fabian M, Sedlák M

机构信息

Institute of Experimental Physics, Slovak Academy of Sciences, Kosice, Czechoslovakia.

出版信息

Biochim Biophys Acta. 1990 Oct 24;1020(1):94-100. doi: 10.1016/0005-2728(90)90098-o.

DOI:10.1016/0005-2728(90)90098-o

PMID:2171645

Abstract

Isolated bovine heart cytochrome oxidase has been extracted into n-hexane, probably in reverse micelles, by the use of asolectin and calcium. The diluted extracts are composed of particles with the hydrodynamic radius of 42 nm. Spectral characteristics of the extracted oxidase are similar to those in aqueous solutions. At the high molar ratio of water to phospholipid (W0 = 8) in an organic solvent both cytochrome a and a3 are reducible and oxygen uptake is observed. However, at low W0 (W0 = 1.8) the rate of cytochrome a reduction is decreased and reduction of cytochrome a3 is inhibited.

摘要

通过使用大豆卵磷脂和钙，已将分离的牛心细胞色素氧化酶提取到正己烷中，可能是形成了反胶束。稀释后的提取物由流体动力学半径为42纳米的颗粒组成。提取的氧化酶的光谱特征与水溶液中的相似。在有机溶剂中，当水与磷脂的摩尔比很高（W0 = 8）时，细胞色素a和a3均可被还原，并观察到氧气摄取。然而，在低W0（W0 = 1.8）时，细胞色素a的还原速率降低，细胞色素a3的还原受到抑制。