Yang Tingting, Lyons Shawn, Aguilar Carmen, Cuhel Russell, Teske Andreas
Department of Marine Sciences, University of North Carolina at Chapel Hill Chapel Hill, NC, USA.
Front Microbiol. 2011 Jun 13;2:130. doi: 10.3389/fmicb.2011.00130. eCollection 2011.
Five sublacustrine thermal spring locations from 1 to 109 m water depth in Yellowstone Lake were surveyed by 16S ribosomal RNA gene sequencing in relation to their chemical composition and dark CO(2) fixation rates. They harbor distinct chemosynthetic bacterial communities, depending on temperature (16-110°C) and electron donor supply (H(2)S <1 to >100 μM; NH(3) <0.5 to >10 μM). Members of the Aquificales, most closely affiliated with the genus Sulfurihydrogenibium, are the most frequently recovered bacterial 16S rRNA gene phylotypes in the hottest samples; the detection of these thermophilic sulfur-oxidizing autotrophs coincided with maximal dark CO(2) fixation rates reaching near 9 μM C h(-1) at temperatures of 50-60°C. Vents at lower temperatures yielded mostly phylotypes related to the mesophilic gammaproteobacterial sulfur oxidizer Thiovirga. In contrast, cool vent water with low chemosynthetic activity yielded predominantly phylotypes related to freshwater Actinobacterial clusters with a cosmopolitan distribution.
通过16S核糖体RNA基因测序，对黄石湖1至109米水深的5个湖底热泉位置进行了调查，分析了其化学成分和暗CO₂固定率。这些热泉拥有不同的化学合成细菌群落，这取决于温度（16 - 110°C）和电子供体供应情况（H₂S含量从小于1 μM到大于100 μM；NH₃含量从小于0.5 μM到大于10 μM）。在最热的样本中，与硫氢基菌属关系最为密切的嗜水生菌目成员，是最常检测到的细菌16S rRNA基因系统发育型；在50 - 60°C的温度下，这些嗜热硫氧化自养菌的检测结果与接近9 μM C h⁻¹的最大暗CO₂固定率相吻合。较低温度的喷口产生的主要是与嗜温γ-变形菌硫氧化菌硫弧菌相关的系统发育型。相比之下，化学合成活性较低的冷喷口水主要产生与具有全球分布的淡水放线菌簇相关的系统发育型。
