中耳胆脂瘤中 EGFR 的表达和微血管密度。
Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma.
机构信息
Department of Otolaryngology-Head and Neck Surgery, Hanyang University College of Medicine, Seoul, Korea.
出版信息
Clin Exp Otorhinolaryngol. 2011 Jun;4(2):67-71. doi: 10.3342/ceo.2011.4.2.67. Epub 2011 May 31.
OBJECTIVES
Cholesteatoma destructs bony tissue by the interactions between hyperproliferative epithelial cells and subepithelial inflammatory cells. The aim of this study was to evaluate the expression of epidermal growth factor receptor (EGFR) and microvessel density (MVD) in middle ear cholesteatoma tissue in an effort to determine the relationship between expression of EGFR and neovascularization.
METHODS
We evaluated the expression of EGFR and MVD by immunohistochemical staining for CD31 and Factor VIII in 32 cholesteatoma tissue samples and 7 normal postauricular skin samples. We also analyzed the correlation between EGFR expression and MVD.
RESULTS
The expression of EGFR was higher in cholesteatoma than in postauricular skin, but the difference was not statistically significant. EGFR was more highly expressed in the suprabasal layer than in the basal layer. Using CD31 and Factor VIII, we analyzed the MVD and found that it was significantly higher in cholesteatoma than in postauricular skin, and significantly correlated with the expression of EGFR.
CONCLUSION
Our results suggest that overexpression of EGFR and neovascularization are correlated with the growth of cholesteatoma.
目的
胆脂瘤通过过度增殖的上皮细胞与上皮下炎症细胞的相互作用破坏骨组织。本研究旨在评估中耳胆脂瘤组织中表皮生长因子受体（EGFR）和微血管密度（MVD）的表达，以确定 EGFR 表达与新生血管形成之间的关系。
方法
我们通过免疫组织化学染色 CD31 和因子 VIII 评估了 32 例胆脂瘤组织样本和 7 例正常耳后皮肤样本中 EGFR 和 MVD 的表达。我们还分析了 EGFR 表达与 MVD 之间的相关性。
结果
胆脂瘤中 EGFR 的表达高于耳后皮肤，但差异无统计学意义。EGFR 在棘细胞上层的表达高于基底层。使用 CD31 和因子 VIII，我们分析了 MVD，发现胆脂瘤中的 MVD 明显高于耳后皮肤，并且与 EGFR 的表达显著相关。
结论
我们的结果表明，EGFR 的过度表达和新生血管形成与胆脂瘤的生长相关。
Zotero 插件