Ergün S, Zheng X, Carlsöö B
Department of Otorhinolaryngology, University Hospital, Karolinska Institute, Huddinge, Sweden.
Am J Otol. 1996 May;17(3):393-6.
To study the possible autocrine growth stimulation of cholesteatoma epithelium, the expression of transforming growth factor alpha (TGF-alpha) and epidermal growth factor receptor (EGFR) in middle ear cholesteatoma was investigated by immunohistochemical staining techniques. Twenty cholesteatoma samples obtained at operation and six normal skin specimens collected from the external ear canal were used in the study. Immunostaining for TGF-alpha showed a diffuse cytoplasmic staining pattern. It was weakly expressed in the basal layer of the normal epidermal epithelium but was more strongly expressed in all cholesteatoma specimens. EGFR showed a dot-like/diffuse cytoplasmic and cell membrane staining pattern. EGFR-positive cells were seen in the basal layer of normal epidermis. In the cholesteatoma specimens, expression of EGFR was not only confined to the basal layer but persisted into the upper layers of the epithelium. Our findings indicate that an autocrine growth stimulation by TGF-alpha and EGFR may contribute to the unrestrained growth of cholesteatoma epithelium in the middle ear cavity.
为研究胆脂瘤上皮细胞可能的自分泌生长刺激作用，采用免疫组织化学染色技术，对外中耳胆脂瘤中转化生长因子α（TGF-α）和表皮生长因子受体（EGFR）的表达进行了研究。本研究使用了20例手术中获取的胆脂瘤样本以及6例从外耳道采集的正常皮肤标本。TGF-α免疫染色显示弥漫性胞质染色模式。它在正常表皮上皮的基底层中弱表达，但在所有胆脂瘤标本中表达更强。EGFR显示点状/弥漫性胞质和细胞膜染色模式。在正常表皮的基底层可见EGFR阳性细胞。在胆脂瘤标本中，EGFR的表达不仅局限于基底层，还持续到上皮的上层。我们的研究结果表明，TGF-α和EGFR的自分泌生长刺激可能促成中耳腔内胆脂瘤上皮细胞的无节制生长。
