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通过高效液相色谱法对羊奶中的两种含岩藻糖的五糖进行纯化,并通过高场¹H核磁共振光谱法对其进行表征。

Purification, by high-performance liquid chromatography, and characterization, by high-field 1H-n.m.r. spectroscopy, of two fucose-containing pentasaccharides of goat's milk.

作者信息

Chaturvedi P, Sharma C B

机构信息

Department of Biosciences and Biotechnology, University of Roorkee, India.

出版信息

Carbohydr Res. 1990 Aug 1;203(1):91-101. doi: 10.1016/0008-6215(90)80048-8.

Abstract

Two fucose-containing pentasaccharides were isolated from goat's milk using a Bio-Gel P-4 column, followed by reverse-phase C-18 high-performance liquid chromatography. The structures of the pentasaccharides as characterized by high-field 1H-n.m.r. spectroscopy and enzymatic digestion were found to be [see text] and [see text].

摘要

使用Bio-Gel P-4柱从山羊奶中分离出两种含岩藻糖的五糖,随后进行反相C-18高效液相色谱分析。通过高场1H-核磁共振光谱和酶解确定的五糖结构为[见原文]和[见原文]。

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