Department of Clinical Analysis, Centre for Health Sciences, Federal University of Santa Catarina, Campus Universitário, Trindade, Florianópolis, SC, Brazil.
J Pharm Pharmacol. 2011 Aug;63(8):1091-102. doi: 10.1111/j.2042-7158.2011.01311.x. Epub 2011 Jun 15.
The aim of this study was to investigate the anti-inflammatory effects of the crude hydroalcoholic extract (CHE) isolated from Esenbeckia leiocarpa Engl., and fractions and subfractions derived from it.
Dried E. leiocarpa Engl. bark was macerated and extracted with ethanol to obtain the CHE. The n-hexane, ethyl acetate, aqueous and alkaloid fractions, as well as two alkaloid subfractions (polar and nonpolar) were obtained from the CHE. A preliminary analysis using thin-layer chromatography was performed. Capillary electrophoresis, physical characteristics and spectral data produced by IR analysis and nuclear magnetic resonance (¹H and ¹³C NMR), and mass spectrometry analysis were used to identify and elucidate the structure of the major compounds. Swiss mice were used in a carrageenan-induced pleurisy model. Pro-inflammatory parameters (leukocyte and exudate concentrations, myeloperoxidase and adenosine-deaminase activity, and nitrate/nitrite, interleukin 1β and tumour necrosis factor α levels) were quantified in exudates at 4 h after carrageenan-induced pleurisy in mice.
The dihydrocorynantheol alkaloid was isolated as the majority compound in the CHE, ethyl acetate and alkaloid fractions, and in the polar and nonpolar alkaloid subfractions. The CHE, fractions and subfractions inhibited the increases in leukocyte and exudate concentrations, myeloperoxidase and adenosine-deaminase activity, and nitrite/nitrate, interleukin 1β, and tumour necrosis factor α levels (P<0.05) in the fluid secreted from the pleural cavity of the carrageenan-treated mice.
E. leiocarpa Engl. showed significant in vivo anti-inflammatory action by inhibiting the inflammation caused by carrageenan. This effect may be, in part, due to the dihydrocorynantheol alkaloid, which was identified as the majority compound isolated from E. leiocarpa bark.
本研究旨在探讨从糙叶树（Esenbeckia leiocarpa Engl.）中分离得到的粗水醇提取物（CHE）及其衍生的馏分和亚馏分的抗炎作用。
干燥糙叶树 Engl.树皮用乙醇浸渍并提取，得到 CHE。从 CHE 中得到正己烷、乙酸乙酯、水和生物碱馏分，以及两个生物碱亚馏分（极性和非极性）。采用薄层色谱法进行初步分析。毛细管电泳、IR 分析产生的物理特性和光谱数据（¹H 和 ¹³C NMR）以及质谱分析用于鉴定和阐明主要化合物的结构。采用角叉菜胶诱导胸膜炎模型的瑞士小鼠。在角叉菜胶诱导的胸膜炎后 4 小时，定量检测渗出物中的促炎参数（白细胞和渗出物浓度、髓过氧化物酶和腺苷脱氨酶活性以及硝酸盐/亚硝酸盐、白细胞介素 1β和肿瘤坏死因子 α 水平）。
二氢考利纳醇生物碱是 CHE、乙酸乙酯和生物碱馏分以及极性和非极性生物碱亚馏分中的主要化合物。 CHE、馏分和亚馏分抑制了角叉菜胶处理的小鼠胸腔渗出液中白细胞和渗出物浓度、髓过氧化物酶和腺苷脱氨酶活性以及亚硝酸盐/硝酸盐、白细胞介素 1β和肿瘤坏死因子 α 水平的升高（P<0.05）。
糙叶树 Engl. 通过抑制角叉菜胶引起的炎症表现出显著的体内抗炎作用。这种作用可能部分归因于二氢考利纳醇生物碱，它被鉴定为从糙叶树树皮中分离得到的主要化合物。
