Department of Clinical Analyses, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário-Trindade, 88040-970, Florianopolis, SC, Brazil.
Int Immunopharmacol. 2011 Dec;11(12):1991-9. doi: 10.1016/j.intimp.2011.08.009. Epub 2011 Sep 2.
This study was conducted to investigate the anti-inflammatory efficacy of Esenbeckia leiocarpa against the inflammation caused by the carrageenan using a murine air pouch model.
The effects of the crude hydroalcoholic extract (CHE), fractions (n-hexane (Hex) and ethyl acetate (AcOEt)), subfractions (polar (Pol) and nonpolar (Nonpol)), or isolated compounds (dihydrocorynantheol (DHC) and beta-sitosterol (β-Sit)) isolated from CHE upon leukocytes, exudate, myeloperoxidase (MPO) adenosine-deaminase (ADA), nitrate/nitrite (NO(x)), interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and inhibitory kappa-B-alpha (IκB-α) degradation were evaluated. The CHE, Alk, Pol, Nonpol, DHC and β-Sit, inhibited leukocytes, exudate, MPO and ADA, NO(x), IL-1β, and TNF-α (P<0.05). The Hex and AcOEt fractions inhibited all of the proinflammatory parameters, except the exudate. The compound DHC prevented the IκB-α degradation.
E. leiocarpa possesses important anti-inflammatory properties. These inhibitory effects occurred along with the downregulation of nitric oxide, IL-1β and TNF-α levels. The isolated compounds DHC and β-Sit may be partially responsible for these anti-inflammatory effects.
本研究旨在通过气囊炎动物模型考察对叶山蚂蝗（Esenbeckia leiocarpa）提取物的抗炎作用。
采用气囊炎动物模型，考察对叶山蚂蝗的粗提物（CHE）及其各萃取部位（正己烷（Hex）、乙酸乙酯（AcOEt））、各萃取部位的亚组分（极性（Pol）和非极性（Nonpol））、以及单体化合物（二氢卡瓦胡椒醇（DHC）和β-谷甾醇（β-Sit））对白细胞、渗出液、髓过氧化物酶（MPO）、腺苷脱氨酶（ADA）、硝酸盐/亚硝酸盐（NOx）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和抑制性κB-α（IκB-α）降解的影响。结果： CHE、Alk、Pol、Nonpol、DHC 和 β-Sit 均能抑制白细胞、渗出液、MPO 和 ADA、NOx、IL-1β和 TNF-α的产生（P<0.05）。Hex 和 AcOEt 部位除渗出液外，对其它各炎症因子均有抑制作用。单体化合物 DHC 可抑制 IκB-α 降解。
对叶山蚂蝗具有显著的抗炎活性，其作用机制可能与抑制一氧化氮（NO）、IL-1β和 TNF-α的产生有关。单体化合物 DHC 和β-Sit 可能是其抗炎活性的部分物质基础。
