Instituto de Pesquisa Estudos e Ambiência Científica, Universidade Paranaense, Umuarama, Paraná, Brazil.
Exp Parasitol. 2011 Sep;129(1):5-10. doi: 10.1016/j.exppara.2011.06.008. Epub 2011 Jun 22.
Several studies have demonstrated that the myenteric plexus experiences quantitative and morphometric changes in rats inoculated orally with Toxoplasma gondii. This paper aims to verify if these alterations are also seen when the same animals are inoculated intraperitoneally with the parasite. In order to do that, six Wistar rats (Rattus norvegicus) 60 days of age were infected intraperitoneally with 10(6) tachyzoites of a genotype I T. gondii strain (BTU IV). After 60 days, the animals were anaesthetised and underwent laparotomy. All organs from the small and large intestines were removed, measured, dissected and underwent whole-mount Giemsa technique to stain the neurons in the myenteric plexus. A quantitative and morphometric analysis of these cells was made, and it showed that the parasite causes the death of myenteric neurons in the jejunum and morphometric alterations in these cells throughout the intestine. However, the cellular response of myenteric neurons to T. gondii is heterogeneous compared the different organs from the gut.
几项研究表明，感染刚地弓形虫的大鼠的肌间神经丛经历了数量和形态计量的变化。本文旨在验证当同一动物经腹腔接种寄生虫时是否也会出现这些变化。为此，将 6 只 60 天大的 Wistar 大鼠（Rattus norvegicus）经腹腔接种 10（6）个 I 型刚地弓形虫（BTU IV）株的速殖子。60 天后，对动物进行麻醉并进行剖腹手术。从小肠和大肠中取出所有器官，进行测量、解剖并进行全组织 Giemsa 染色以显示肌间神经丛中的神经元。对这些细胞进行了定量和形态计量分析，结果表明寄生虫导致空肠肌间神经元死亡，并在整个肠道中导致这些细胞的形态计量改变。然而，与肠道的不同器官相比，肌间神经元对刚地弓形虫的细胞反应是异质的。
