Papazian-Cabanas Rodrigo M, Araújo Eduardo J A, Silva Aristeu V da, Sant'Ana Débora M G
Universidade Paranaense, Umuarama, PR, Brasil.
An Acad Bras Cienc. 2012 Sep;84(3):737-46. doi: 10.1590/s0001-37652012005000052. Epub 2012 Jul 24.
The effects of acute and chronic infection caused by Toxoplasma gondii on duodenal myenteric neurons were analyzed. Eighteen rats were assigned into four groups: Acute Control Group (ACG, n=4); Acute Experimental Group (AEG, n=4); Chronic Control Group (CCG, n=5); and Chronic Experimental Group (CEG, n=5). Rats from the AEG and CEG were inoculated orally with 105 genotype III (BTU-II strain) tachyzoites of T. gondii isolated from a dog with neurological signs. Acute groups were killed after 24 hours after the inoculation and the chronic groups after 30 days. Whole-mount from the duodenum were stained with Giemsa. The population density of myenteric neurons, as well the body cell, nuclear and cytoplasmic area were analyzed. Both acute and chronic toxoplasmic infection did not provoke neuronal loss. On the other hand, plastic alterations were observed: decreasing of the nuclear and cytoplasmic area during the acute phase and neuronal hypertrophy during the chronic phase.
分析了刚地弓形虫急性和慢性感染对十二指肠肌间神经元的影响。将18只大鼠分为四组：急性对照组（ACG，n = 4）；急性实验组（AEG，n = 4）；慢性对照组（CCG，n = 5）；慢性实验组（CEG，n = 5）。AEG组和CEG组大鼠经口接种从一只患有神经症状的狗身上分离出的105个刚地弓形虫III型（BTU-II株）速殖子。接种后24小时处死急性组，30天后处死慢性组。十二指肠整装片用吉姆萨染色。分析肌间神经元的群体密度以及体细胞、细胞核和细胞质面积。急性和慢性弓形虫感染均未引起神经元丢失。另一方面，观察到可塑性改变：急性期细胞核和细胞质面积减小，慢性期神经元肥大。
