刚地弓形虫可导致空肠肌间神经丛的死亡和形态改变。
Toxoplasma gondii causes death and plastic alteration in the jejunal myenteric plexus.
作者信息
Araújo Eduardo José de Almeida, Zaniolo Larissa Marchi, Vicentino Suellen Laís, Góis Marcelo Biondaro, Zanoni Jacqueline Nelisis, da Silva Aristeu Vieira, Sant'Ana Débora de Mello Gonçales
机构信息
Eduardo José de Almeida Araújo, Departamento de Histologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86057-970 Paraná, Brazil.
出版信息
World J Gastroenterol. 2015 Apr 28;21(16):4829-39. doi: 10.3748/wjg.v21.i16.4829.
AIM
To assess the effects of ME-49 Toxoplasma gondii (T. gondii) strain infection on the myenteric plexus and external muscle of the jejunum in rats.
METHODS
Thirty rats were distributed into two groups: the control group (CG) (n = 15) received 1 mL of saline solution orally, and the infected group (IG) (n = 15) inoculated with 1 mL of saline solution containing 500 oocysts of M-49 T. gondii strain orally. After 36 d of infection, the rats were euthanized. Infection with T. gondii was confirmed by blood samples collected from all rats at the beginning and end of the experiment. The jejunum of five animals was removed and submitted to routine histological processing (paraffin) for analysis of external muscle thickness. The remaining jejunum from the others animals was used to analyze the general population and the NADH-diaphorase, VIPergic and nitrergic subpopulations of myenteric neurons; and the enteric glial cells (S100-IR).
RESULTS
Serological analysis showed that animals from the IG were infected with the parasite. Hypertrophy affecting jejunal muscle thickness was observed in the IG rats (77.02 ± 42.71) in relation to the CG (51.40 ± 12.34), P < 0.05. In addition, 31.2% of the total number of myenteric neurons died (CG: 39839.3 ± 5362.3; IG: 26766.6 ± 2177.6; P < 0.05); hyperplasia of nitrergic myenteric neurons was observed (CG: 7959.0 ± 1290.4; IG: 10893.0 ± 1156.3; P < 0.05); general hypertrophy of the cell body in the remaining myenteric neurons was noted [CG: 232.5 (187.2-286.0); IG: 248.2 (204.4-293.0); P < 0.05]; hypertrophy of the smallest varicosities containing VIP neurotransmitter was seen (CG: 0.46 ± 0.10; IG: 0.80 ± 0.16; P < 0.05) and a reduction of 25.3% in enteric glia cells (CG: 12.64 ± 1.27; IG: 10.09 ± 2.10; P < 0.05) was observed in the infected rats.
CONCLUSION
It was concluded that infection with oocysts of ME-49 T. gondii strain caused quantitative and plastic alterations in the myenteric plexus of the jejunum in rats.
目的
评估ME-49株刚地弓形虫感染对大鼠空肠肌间神经丛和外肌的影响。
方法
将30只大鼠分为两组：对照组（CG）（n = 15）口服1 mL生理盐水，感染组（IG）（n = 15）口服1 mL含500个ME-49株刚地弓形虫卵囊的生理盐水。感染36天后，将大鼠安乐死。在实验开始和结束时从所有大鼠采集血样以确认刚地弓形虫感染。取出5只动物的空肠并进行常规组织学处理（石蜡包埋）以分析外肌厚度。其余动物的空肠用于分析肌间神经元的总体以及烟酰胺腺嘌呤二核苷酸黄递酶、血管活性肠肽能和一氧化氮能亚群；以及肠胶质细胞（S100免疫反应性）。
结果
血清学分析表明IG组动物感染了该寄生虫。与CG组（51.40±12.34）相比，IG组大鼠空肠肌厚度出现肥大（77.02±42.71），P<0.05。此外，肌间神经元总数的31.2%死亡（CG组：39839.3±5362.3；IG组：26766.6±2177.6；P<0.05）；观察到一氧化氮能肌间神经元增生（CG组：7959.0±1290.4；IG组：10893.0±1156.3；P<0.05）；其余肌间神经元的细胞体普遍肥大[CG组：232.5（187.2 - 286.0）；IG组：248.2（204.4 - 293.0）；P<0.05]；观察到含有血管活性肠肽神经递质的最小膨体肥大（CG组：0.46±0.10；IG组：0.80±0.16；P<0.05），并且感染大鼠的肠胶质细胞减少了25.3%（CG组：12.64±1.27；IG组：10.09±2.10；P<0.05）。
结论
得出结论，ME-49株刚地弓形虫卵囊感染导致大鼠空肠肌间神经丛发生数量和形态改变。
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