Zoiray Technologies Inc., Boston, MA, USA.
J Immunol Methods. 2011 Aug 31;371(1-2):159-64. doi: 10.1016/j.jim.2011.06.004. Epub 2011 Jun 22.
A dual detection system for protein arrays is presented that combines label-free detection by optical interference with chemiluminescence. A planar protein array that targets hepatitis B surface antigen is developed. Surface densities for individual antibody spots are quantitated using optical interference prior to use. Target binding (10 ng/ml) is detected label-free. Target binding (1 ng/ml) is detected by both optical interference and chemiluminescence with the inclusion of secondary antibodies. Binding results using both methods are found to be directly proportion to the capture probe density measured initially. The dual detection system provides the analytical utility of optical interference detection with the established clinical utility of chemiluminescence detection.
本文提出了一种用于蛋白质芯片的双重检测系统，该系统结合了免标记光学干涉检测和化学发光检测。开发了一种针对乙型肝炎表面抗原的平面蛋白质芯片。在使用前，使用光学干涉对单个抗体斑点的表面密度进行定量。通过免标记检测到靶结合（10ng/ml）。通过包含二级抗体，通过光学干涉和化学发光均可检测到靶结合（1ng/ml）。两种方法的结合结果都发现与最初测量的捕获探针密度成正比。该双重检测系统提供了光学干涉检测的分析效用，以及化学发光检测的既定临床效用。
