双脱氧福司可林对鸡胚软骨细胞培养物中蛋白聚糖合成及结构的影响。

Effects of dideoxyforskolin on proteoglycan synthesis and structure in embryonic chick chondrocyte cultures.

作者信息

Hu L M, Kemp S F, Peng C F, Elders M J, Smith W G

机构信息

Department of Biochemistry, University of Arkansas for Medical Sciences, Little Rock 72205.

出版信息

FEBS Lett. 1990 Sep 17;270(1-2):100-4. doi: 10.1016/0014-5793(90)81243-h.

DOI:10.1016/0014-5793(90)81243-h

PMID:2171982

Abstract

1,9-Dideoxyforskolin inhibits proteoglycan synthesis and xyloside-initiated glycosaminoglycan (GAG) synthesis in chick embryo chondrocytes. Dideoxyforskolin does not affect the length of xyloside-initiated GAG chains secreted into the medium but chains from the dense proteoglycan secreted into the medium appear slightly longer. Incorporation of labeled serine into the dense proteoglycan and subsequent digestion with Pronase revealed a dramatic decrease in percent of total radioactivity associated with GAG chains in the proteoglycan from cultures treated with forskolin or dideoxyforskolin. These observations suggest that these diterpenes have a specific inhibitory effect on chain initiation reactions and thus may be useful tools in the study of proteoglycan synthesis and processing.

摘要

1,9-二脱氧佛司可林抑制鸡胚软骨细胞中蛋白聚糖的合成以及木糖苷引发的糖胺聚糖（GAG）合成。二脱氧佛司可林不影响分泌到培养基中的木糖苷引发的GAG链的长度，但分泌到培养基中的致密蛋白聚糖的链似乎略长。将标记的丝氨酸掺入致密蛋白聚糖中，随后用链霉蛋白酶消化，结果显示，在用佛司可林或二脱氧佛司可林处理的培养物中，与蛋白聚糖中GAG链相关的总放射性百分比显著降低。这些观察结果表明，这些二萜对链起始反应具有特异性抑制作用，因此可能是研究蛋白聚糖合成和加工的有用工具。