福司可林对鸡胚软骨细胞中木糖苷引发的糖胺聚糖合成的影响。

Effect of forskolin on synthesis of xyloside-initiated glycosaminoglycans in embryonic chick chondrocytes.

作者信息

Hu L M, Kemp S F, Elders M J, Smith W G

机构信息

Department of Biochemistry/Molecular Biology, University of Arkansas for Medical Science, Little Rock.

出版信息

Biochim Biophys Acta. 1990 Jan 23;1051(1):112-4. doi: 10.1016/0167-4889(90)90181-c.

DOI:10.1016/0167-4889(90)90181-c

PMID:2297536

Abstract

The synthesis of sulfated glycosaminoglycan (GAG) chains has been studied in the presence of various concentrations of the artificial acceptor 4-methylumbelliferyl beta-D-xyloside and of forskolin. Sulfated GAG chains formed in the presence of forskolin had a smaller hydrodynamic radius than controls, as revealed by chromatography on Sepharose CL-6B. Sulfated GAGs from both control and treated cultures behaved identically when chromatographed on DEAE.

摘要

在存在不同浓度的人工受体4-甲基伞形酮基β-D-木糖苷和福斯高林的情况下，对硫酸化糖胺聚糖（GAG）链的合成进行了研究。如通过琼脂糖凝胶CL-6B色谱分析所揭示的，在福斯高林存在下形成的硫酸化GAG链的流体力学半径比对照的小。当在DEAE上进行色谱分析时，来自对照培养物和处理培养物的硫酸化GAG表现相同。